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Intragenomic Polymorphism of the ITS 1 Region of 35S rRNA Gene in the Group of Grasses with Two-Chromosome Species: Different Genome Composition in Closely Related Zingeria Species
Plants ( IF 4.658 ) Pub Date : 2020-11-25 , DOI: 10.3390/plants9121647
Alexander V. Rodionov , Alexander A. Gnutikov , Nikolai N. Nosov , Eduard M. Machs , Yulia V. Mikhaylova , Victoria S. Shneyer , Elizaveta O. Punina

Zingeria (Poaceae) is a small genus that includes Z. biebersteiniana, a diploid species with the lowest chromosome number known in plants (2n = 4) as well as hexaploid Z. kochii and tetraploid Z. pisidica, and/or Z. trichopoda species. The relationship between these species and the other low-chromosomes species Colpodium versicolor are unclear. To explore the intragenomic polymorphism and genome composition of these species we examined the sequences of the internal transcribed spacer 1 of the 35S rRNA gene via NGS approach. Our study revealed six groups of ribotypes in Zingeria species. Their distribution confirmed the allopolyploid nature of Z. kochii, whose probable ancestors were Colpodium versicolor and Z. pisidica. Z. pisidica has 98% of rDNA characteristic only for this species, and about 0.3% of rDNA related to that of Z. biebersteiniana. We assume that hexaploid Z. kochii is either an old allopolyploid or a homodiploid that has lost most of the rRNA genes obtained from Z. biebersteiniana. In Z. trichopoda about 81% of rDNA is related to rDNA of Z. biebersteiniana and 19% of rDNA is derived from Poa diaphora sensu lato. The composition of the ribotypes of the two plants determined by a taxonomy specialist as Z. pisidica and Z. trichopoda is very different. Two singleton species are proposed on this base with ribotypes as discriminative characters. So, in all four studied Zingeria species, even if the morphological difference among the studied species was modest, the genomic constitution was significantly different, which suggests that these are allopolyploids that obtained genomes from different ancestors.

中文翻译:

具有两个染色体物种的草群中35S rRNA基因ITS 1区的Intragenomic多态性:密切相关的姜属物种中不同的基因组组成

姜黄属(禾本科)是一种小属,包括Z. biebersteiniana,一种在植物中已知的最低染色体数的二倍体物种(2n = 4),以及六倍体Z. kochii和四倍体Z. pisidica,和/或Trichopoda物种。这些物种和其他低染色体物种杂色粉虱之间的关系尚不清楚。为了探索这些物种的基因组内多态性和基因组组成,我们通过NGS方法检查了35S rRNA基因内部转录间隔区1的序列。我们的研究揭示了Zingeria物种中的六种核糖型。它们的分布证实了Z. kochii的异源多倍体性质,其可能的祖先是云芝粉和Z. pisidica。pisidica Z. pisidica仅对该物种具有98%的rDNA特征,而与biebersteinsteiniana的rDNA相关的rDNA约为0.3%。我们假设六倍体Z. kochii是一个古老的异源多倍体或同二倍体,已经失去了从Z. biebersteiniana获得的大多数rRNA基因的能力。在Trichopoda中,约81%的rDNA与Z. biebersteiniana的rDNA相关,而19%的rDNA则来自于印度洋槐。由分类学专家确定的两种植物的核糖型组成分别为Z. pisidica和Z. trichopoda。在此基础上提出了两个单核种,以核糖型为判别特征。因此,在所有四个研究的姜氏菌属物种中,即使所研究的物种之间的形态差异不大,其基因组组成也显着不同,这表明它们是从不同祖先获得基因组的同种多倍体。kochii是一种古老的异源多倍体或同二倍体,已经失去了从Z. biebersteiniana获得的大部分rRNA基因。在Trichopoda中,约81%的rDNA与Z. biebersteiniana的rDNA相关,而19%的rDNA则来自于印度洋槐。由分类学专家确定的两种植物的核糖型组成分别为Z. pisidica和Z. trichopoda。在此基础上提出了两个单核种,以核糖型为判别特征。因此,在所有四个研究的姜氏菌属物种中,即使所研究的物种之间的形态差异不大,其基因组组成也显着不同,这表明它们是从不同祖先获得基因组的同种多倍体。kochii是一种古老的异源多倍体或同二倍体,已经失去了从Z. biebersteiniana获得的大部分rRNA基因。在Trichopoda中,约81%的rDNA与Z. biebersteiniana的rDNA相关,而19%的rDNA则来自于印度洋槐。由分类学专家确定的两种植物的核糖型组成分别为Z. pisidica和Z. trichopoda。在此基础上提出了两个单核种,以核糖型为判别特征。因此,在所有四个研究的姜氏菌属物种中,即使所研究的物种之间的形态差异不大,其基因组组成也显着不同,这表明它们是从不同祖先获得基因组的同种多倍体。毛滴虫中,约有81%的rDNA与比伯氏菌(Z. biebersteiniana)的rDNA有关,而约19%的rDNA则来自印度洋槐(Poa diaphora sensu lato)。由分类学专家确定的两种植物的核糖型组成分别为Z. pisidica和Z. trichopoda。在此基础上提出了两个单核种,以核糖型为判别特征。因此,在所有四个研究的姜氏菌属物种中,即使所研究的物种之间的形态差异不大,其基因组组成也显着不同,这表明它们是从不同祖先获得基因组的同种多倍体。毛滴虫中,约有81%的rDNA与比伯氏菌(Z. biebersteiniana)的rDNA有关,而约19%的rDNA则来自印度洋槐(Poa diaphora sensu lato)。由分类学专家确定的两种植物的核糖型组成分别为Z. pisidica和Z. trichopoda。在此基础上提出了两个单核种,以核糖型为判别特征。因此,在所有四个研究的姜氏菌属物种中,即使所研究的物种之间的形态差异不大,其基因组组成也显着不同,这表明它们是从不同祖先获得基因组的同种多倍体。滴虫非常不同。在此基础上提出了两个单核种,以核糖型为判别特征。因此,在所有四个研究的姜氏菌属物种中,即使所研究的物种之间的形态差异不大,其基因组组成也显着不同,这表明它们是从不同祖先获得基因组的同种多倍体。滴虫非常不同。在此基础上提出了两个单核种,以核糖型为判别特征。因此,在所有四个研究的姜氏菌属物种中,即使所研究的物种之间的形态差异不大,其基因组组成也显着不同,这表明它们是从不同祖先获得基因组的同种多倍体。
更新日期:2020-11-25
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