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Effect of AuNPs and AgNPs on the Antioxidant System and Antioxidant Activity of Lavender (Lavandula angustifolia Mill.) from In Vitro Cultures
Molecules ( IF 4.6 ) Pub Date : 2020-11-25 , DOI: 10.3390/molecules25235511
Paula Jadczak , Danuta Kulpa , Radosław Drozd , Włodzimierz Przewodowski , Agnieszka Przewodowska

The aim of this study was to determine the effect of gold and silver nanoparticles on the activity of antioxidant enzymes (ascorbate peroxidase (APX), superoxide dismutase (SOD), guaiacol peroxidase (POX), and catalase (CAT)), the free radical scavenging capacity, and the total polyphenol capacity of lavender (Lavandula angustifolia Mill.) cultivar “Munstead” propagated in vitro. In the experiment, fragments of lavender plants were cultivated in vitro on medium with the addition of 1, 2, 5, 10, 20, and 50 mg∙dm−3 of AgNPs or AuNPs (particle sizes 24.2 ± 2.4 and 27.5 ± 4.8 nm, respectively). It was found that the nanoparticles increase the activity of the antioxidant enzymes APX and SOD; however, the reaction depends on the NP concentration. The highest APX activity is found in plants propagated on media with 2 and 5 mg∙dm−3 of AgNPs. AuNPs significantly increase the APX activity when added to media with a concentration of 10 mg∙dm−3. The highest SOD activity is recorded at 2 and 5 mg∙dm−3 AgNP and AuNP concentrations. The addition of higher concentrations of nanoparticles to culture media results in a decrease in the APX and SOD activity. The addition of AuNPs to culture media at concentrations from 2 to 50 mg∙dm−3 increases the POX activity in comparison to its activity when AgNPs are added to the culture media. No significant influence of NPs on the increase in CAT activity was demonstrated. AgNPs and AuNPs increased the free radical scavenging capacity (ABTS•+). The addition of NPs at concentrations of 2 and 5 mg∙dm−3 increased the production of polyphenols; however, in lower concentrations it decreased their content in lavender tissues.

中文翻译:

AuNPs 和 AgNPs 对体外培养的薰衣草 (Lavandula angustifolia Mill.) 的抗氧化系统和抗氧化活性的影响

本研究的目的是确定金和银纳米颗粒对抗氧化酶(抗坏血酸过氧化物酶 (APX)、超氧化物歧化酶 (SOD)、愈创木酚过氧化物酶 (POX) 和过氧化氢酶 (CAT))活性的影响。清除能力,以及体外繁殖的薰衣草 (Lavandula angustifolia Mill.) 品种“Munstead”的总多酚能力。在实验中,薰衣草植物的碎片在添加 1、2、5、10、20 和 50 mg∙dm-3 的 AgNPs 或 AuNPs(粒径 24.2 ± 2.4 和 27.5 ± 4.8 nm)的培养基上体外培养, 分别)。发现纳米颗粒增加了抗氧化酶APX和SOD的活性;然而,反应取决于 NP 浓度。在含有 2 和 5 mg∙dm-3 AgNPs 的培养基上繁殖的植物中发现 APX 活性最高。当以 10 mg∙dm-3 的浓度添加到培养基中时,AuNPs 显着增加 APX 活性。最高的 SOD 活性记录在 2 和 5 mg∙dm-3 AgNP 和 AuNP 浓度下。向培养基中添加更高浓度的纳米颗粒会导致 APX 和 SOD 活性降低。与将 AgNPs 添加到培养基中时的活性相比,将 AuNPs 以 2 到 50 mg∙dm-3 的浓度添加到培养基中会增加 POX 活性。NPs 对 CAT 活性的增加没有显着影响。AgNPs 和AuNPs 增加了自由基清除能力(ABTS•+)。添加浓度为 2 和 5 mg∙dm−3 的 NPs 增加了多酚的产量;然而,在较低浓度下,它降低了它们在薰衣草组织中的含量。
更新日期:2020-11-25
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