We herein propose an easy-to-prepare mini-chemosensor array constructed by two-types of off-the-shelf coumarin dyes for simultaneous classification and quantification of sulfur-containing amino acids (SCAAs) (i.e., l-cysteine, l-cystine, l-homocysteine, glutathione, and glutathione disulfide). The detection mechanism of SCAAs relied on a coordination-based sensor array (CBSA) utilizing the competitive binding among the coumarin dye, a Zn²⁺ ion and SCAAs. The reversible feature of the coordination bond of the coumarin-Zn²⁺ resulted in UV/vis spectral shifts and fluorescence enhancement in response to the analytes, offering the multianalyte detection in high accuracy. Furthermore, a mixture of glutathione and l-cysteine was successfully quantified in a sample containing human blood serum. This study would be an important example of the optical chemosensor array toward the rapid and accurate detection of biomarkers.

中文翻译：

---

易于制备的微型化学传感器阵列，用于同时检测半胱氨酸和谷胱甘肽衍生物

我们在此提出了一种易于制备的微型化学传感器阵列，由两种现成的香豆素染料构成，用于同时分类和定量含硫氨基酸 (SCAA)（即l-半胱氨酸、l-胱氨酸） , l-同型半胱氨酸、谷胱甘肽和谷胱甘肽二硫化物)。SCAAs 的检测机制依赖于基于配位的传感器阵列 (CBSA)，利用香豆素染料、Zn ²⁺离子和 SCAAs 之间的竞争性结合。^{香豆素-Zn 2+}的配位键的可逆特性导致响应分析物的紫外/可见光谱偏移和荧光增强，从而提供高精度的多分析物检测。此外，谷胱甘肽和l-半胱氨酸在含有人血清的样品中成功定量。这项研究将成为快速准确检测生物标志物的光学化学传感器阵列的一个重要例子。