Anaerobic oxidation of methane (AOM) coupled with simultaneous consumption of nitrous oxide (N₂O) has been recently reported, but the underlying coupling mechanism remains unclear. Here, we investigated N₂O-mediated AOM in paddy soil under amendment of electron shuttles (anthraquinone-2,6-disulphonate and methylviologen). Sixty-day anaerobic incubation showed that N₂O-mediated AOM occurred in paddy soil, and that electron shuttles stimulated AOM coupled to N₂O reduction. Approximate 60% of ¹³CH₄–C was converted into soil organic matter in paddy soil during anaerobic oxidation. Our results suggest that electron shuttles may play important roles in mitigating the emission of greenhouse gases (CH₄ and N₂O) and increasing organic matter in paddy soils.

最近已经报道了甲烷的厌氧氧化（AOM）以及同时消耗一氧化二氮（N ₂ O），但是潜在的耦合机理仍不清楚。在这里，我们研究了在电子穿梭物质（蒽醌-2,6-二磺酸盐和甲基紫精）的修饰下水稻土中N ₂ O介导的AOM。六十天厌氧培养表明，N ₂ O介导的AOM发生在稻田土壤中，并且电子穿梭刺激了AOM与N ₂ O的还原反应。¹³ CH _4的大约60％在厌氧氧化过程中，-C在稻田土壤中转化为土壤有机质。我们的研究结果表明，电子穿梭可能在减轻温室气体（CH ₄和N ₂ O）的排放以及增加稻田土壤有机质方面发挥重要作用。