Troxerutin (Tx), known as vitamin P₄ is a derivative of natural bioflavonoid rutin. Tx possesses different biological activities such as antioxidant, anticancer, and anti-inflammatory. The current study was conducted to determine potential therapeutic effect of Tx in lipopolysaccharides (LPS)-induced sepsis in mice. In LPS-induced sepsis, the mice were treated intraperitoneally (ip) with Tx twice daily. Therapeutic effect was assessed by measuring serum level of cytokines, alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Level of nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT), Myeloperoxidase (MPO) and Malondialdehyde (MDA) was measured. Expression of CD40 receptor on leucocytes was measured using flowcytometry. Splenocyte proliferation was evaluated using MTT assay. The effect of Tx on survival rate during administration of lethal dose of LPS was investigated. The results showed that Tx inhibited LPS induced NO production. Inflammatory pathways were suppressed by reduction of inflammatory cytokines production. Further, elevated CD40 expression of leucocytes and proliferation of splenocytes markedly reduced in Tx treated group. Antioxidant defense system was enhanced by increased activity of SOD and CAT and decreased level of MDA. MPO, ALT and LDH activity. Additionally, treatment with Tx significantly increased the mean survival time of mice compared with the LPS treated group. Histologically, Tx treatment decreased inflammatory cells infiltration and histopathologicl changes in the liver. Our findings showed that reduced inflammatory parameters, improved antioxidant activity, reduced histological lesions and increased survival rate. These findings suggest that Tx is an effective anti-inflammatory agent for the treatment of LPS-induced sepsis.

Troxerutin（Tx），称为维生素P ₄是天然生物类黄酮芦丁的衍生物。Tx具有不同的生物活性，例如抗氧化剂，抗癌剂和抗炎药。进行当前的研究以确定Tx在脂多糖（LPS）诱导的小鼠败血症中的潜在治疗作用。在LPS诱导的脓毒症中，每天两次用Tx腹膜内（ip）治疗小鼠。通过测量血清中细胞因子，丙氨酸转氨酶（ALT）和乳酸脱氢酶（LDH）的水平来评估治疗效果。测量一氧化氮（NO），超氧化物歧化酶（SOD），过氧化氢酶（CAT），髓过氧化物酶（MPO）和丙二醛（MDA）的水平。使用流式细胞术测量CD40受体在白细胞上的表达。使用MTT测定法评估脾细胞增殖。研究了在致死剂量的LPS给药过程中Tx对存活率的影响。结果表明，Tx抑制LPS诱导的NO产生。通过减少炎性细胞因子的产生来抑制炎性途径。此外，在Tx处理组中，白细胞的CD40表达升高和脾细胞的增殖明显减少。抗氧化防御系统通过增加SOD和CAT的活性以及降低MDA的水平来增强。MPO，ALT和LDH活性。另外，与LPS治疗组相比，Tx治疗显着增加了小鼠的平均存活时间。从组织学上讲，Tx治疗减少了肝脏中炎性细胞的浸润和组织病理学变化。我们的研究结果表明，炎症参数降低，抗氧化活性提高，减少组织学病变并提高生存率。这些发现表明，Tx是用于治疗LPS诱导的败血症的有效抗炎剂。