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Catechin Gallate Acts as a Key Metabolite Induced by Trypsin in Hylocereus undatus during Storage Indicated by Omics
Plant Physiology and Biochemistry ( IF 6.5 ) Pub Date : 2020-11-25 , DOI: 10.1016/j.plaphy.2020.11.036
Xinyue Pang , Shoujing Zhao , Min Zhang , Luning Cai , Yinyin Zhang , Xin Li

Trypsin is a novel superoxide scavenger. The storage quality of H. undatus was significantly improved by trypsin. To investigate the mechanism of flavonoid metabolism regulated by trypsin, combined analysis of widely targeted metabolomic and transcriptome were performed. GO and KEGG enrichment analyses of the transcriptome profiles of H. undatus revealed that some of the flavonoid related biosynthesis pathways were regulated by up or down patterns with the treatment of trypsin. Correlation analysis of flavonoid related genes expression in H. undatus provided a rationale for the functional significance of them. Furthermore, it has been revealed that the most significantly regulated flavonoid was catechin gallate in metabolomic profiles of H. undatus. The major route of flavonoid biosynthesis regulated by trypsin was also illustrated by both transcriptomic and metabolomic data. Finally, the results of PPI network revealed that C4H, HCT, and CYP75B1 acted as hub proteins involved in flavonoid metabolism regulated by trypsin.



中文翻译:

儿茶素没食子酸酯作为一个主要代谢产物乙醛对胰蛋白酶在火龙果贮藏期间组学指示的

胰蛋白酶是一种新型的超氧化物清除剂。胰蛋白酶可显着提高H. undatus的储存质量。为了研究胰蛋白酶调节类黄酮代谢的机制,进行了广泛靶向的代谢组学和转录组分析。GO和KEGG富集的H. undatus转录组谱分析表明,通过胰蛋白酶处理,某些类黄酮相关的生物合成途径受到上调或下调的调控。黄un中黄酮相关基因表达的相关分析为它们的功能意义提供了理论依据。此外,已发现在代谢组学研究中,调节最明显的类黄酮是儿茶素没食子酸酯。H.恩达图斯。转录组和代谢组学数据也说明了受胰蛋白酶调节的类黄酮生物合成的主要途径。最后,PPI网络的结果显示,C4H,HCT和CYP75B1是参与胰蛋白酶调节的类黄酮代谢的枢纽蛋白。

更新日期:2020-11-26
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