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Temporal assessment of root and shoot colonization of elephant grass (Pennisetum purpureum Schum.) host seedlings by Gluconacetobacter diazotrophicus strain LP343
Microbiological Research ( IF 6.7 ) Pub Date : 2020-11-25 , DOI: 10.1016/j.micres.2020.126651
Carlos M Dos-Santos 1 , Wiglison B A Nascimento 2 , Bruna P do Nascimento 3 , Stefan Schwab 1 , José I Baldani 1 , Marcia S Vidal 1
Affiliation  

Gluconacetobacter diazotrophicus is a species of great agronomic potential due to its growth-promotion traits. Its colonization process in different plants has been reported. However, there have been no studies regarding its structural colonization in elephant grass. This is a fast-growing C4-Poaceae plant, and its application in Brazil is mainly aimed at feeding dairy cattle, due to its high nutritional value. Also, in the last decade, this grass has been applied in the production of biofuels. The present study aimed to monitor the colonization process of strain LP343 of G. diazotrophicus inoculated in elephant grass seedlings of PCEA genotype, by using a mCherry-tagged bacterium. Samples of roots and shoots collected at different periods were visualized by confocal laser-scanning microscopy. The colony-counting assay was used to compare the number of cells recovered in different niches and a qPCR was performed for the quantification of endophytic cells in root and shoot tissues. Results suggested that the strain LP343 quickly recognized the PCEA roots as host, attached to the elephant grass roots at 6 h, and 7 days after inoculation were able to colonize the xylem vessels of roots and shoots of elephant grass. This study advances our knowledge about the colonization process of G. diazotrophicus species in elephant grass, contributing to future studies involving the plant-bacteria interaction cultivated under gnotobiotic conditions.



中文翻译:

固氮葡糖醋杆菌菌株 LP343 对象草(Pennisetum purpureum Schum.)寄主幼苗根和枝条定植的时间评估

由于其促进生长的特性,固氮葡萄糖乙酸杆菌是一种具有巨大农艺潜力的物种。已经报道了它在不同植物中的定殖过程。然而,目前还没有关于其在象草中的结构定植的研究。这是一种速生的C4禾本科植物,由于其营养价值高,在巴西的应用主要针对奶牛的饲养。此外,在过去十年中,这种草已用于生产生物燃料。本研究的目的是监测应变LP343的定殖过程G.重氮营养在PCEA基因型的象草苗接种,通过使用mCherry-标记的细菌。通过共聚焦激光扫描显微镜观察在不同时期收集的根和芽样品。菌落计数测定用于比较在不同生态位中回收的细胞数量,并进行 qPCR 以量化根和芽组织中的内生细胞。结果表明,菌株 LP343 迅速​​将 PCEA 根识别为宿主,并在接种后 6 h 和 7 天附着在象草根上,能够定殖象草根和芽的木质部血管。这项研究提高了我们对固氮菌在象草中定植过程的认识,有助于未来涉及在无菌条件下培养的植物-细菌相互作用的研究。

更新日期:2020-12-29
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