As the periodic emission of light pulses by light emitting diodes (LEDs) is known to stimulate growth or induce high value biocompounds in microalgae, this flashing light regime was tested on growth and biochemical composition of the microalgae Nannochloropsis gaditana, Koliella antarctica and Tetraselmis chui. At low flashing light frequencies (e.g., 5 and 50 Hz, Duty cycle = 0.05), a strain-dependent growth inhibition and an accumulation of protein, polyunsaturated fatty acids, chlorophyll or carotenoids (lutein, β-carotene, violaxanthin and neoxanthin) was observed. In addition, a 4-day application of low-frequency flashing light to concentrated cultures increased productivities of eicosapentaenoic acid (EPA) and specific carotenoids up to three-fold compared to continuous or high frequency flashing light (500 Hz, Duty cycle = 0.05). Therefore, applying low-frequency flashing light as finishing step in industrial production can increase protein, polyunsaturated fatty acids or pigment contents in biomass, leading to high-value algal products.

由于已知发光二极管 (LED) 周期性发射光脉冲会刺激微藻的生长或诱导高价值生物化合物，因此对微藻Nannochloropsis gaditana、Koliella antarctica和Tetraselmis chui 的生长和生化成分进行了测试。. 在低闪光频率（例如，5 和 50 Hz，占空比 = 0.05）下，依赖于菌株的生长抑制和蛋白质、多不饱和脂肪酸、叶绿素或类胡萝卜素（叶黄素、β-胡萝卜素、紫黄质和新黄质）的积累是观察到的。此外，与连续或高频闪光灯（500 Hz，占空比 = 0.05）相比，将低频闪光灯应用于浓缩培养物 4 天可将二十碳五烯酸 (EPA) 和特定类胡萝卜素的生产率提高三倍. 因此，在工业生产中应用低频闪光作为精加工步骤，可以增加生物质中蛋白质、多不饱和脂肪酸或色素的含量，从而产生高价值的藻类产品。