Contaminants of emerging concern and ocean changes are key environmental stressors for marine species with possibly synergistic, but still unexplored, deleterious effects. In the present study the influence of a simulated ocean acidification scenario (pH = 7.6) was investigated on metabolism and sub-lethal effects of carbamazepine, CBZ (1 µg/L), chosen as one of the most widely diffused pharmaceuticals in marine organisms. A multidisciplinary approach was applied on mussels, M. galloprovincialis, integrating measurement of drug bioaccumulation with changes in the whole transcriptome, responsiveness of various biochemical and cellular biomarkers including immunological parameters, lipid and oxidative metabolism, onset of genotoxic effects. Chemical analyses revealed a limited influence of hypercapnia on accumulation and excretion of CBZ, while a complex network of biological responses was observed in gene expression profile and functional changes at cellular level. The modulation of gamma-aminobutyric acid (GABA) pathway suggested similarities with the Mechanism of Action known for vertebrates: immune responses, cellular homeostasis and oxidative system represented the processes targeted by combined stressors. The overall elaboration of results through a quantitative Weight of Evidence model, revealed clearly increased cellular hazard due to interactions of CBZ with acidification compared to single stressors.

引起关注的污染物和海洋变化是海洋物种的主要环境压力源，可能产生协同作用，但仍未发掘有害影响。在本研究中，研究了模拟海洋酸化情景（pH = 7.6）对卡马西平CBZ（1 µg / L）的代谢和亚致死作用的影响，CBZ被选为海洋生物中扩散最广泛的药物之一。贻贝，M。galloprovincialis，将药物生物蓄积性的测量与整个转录组的变化，各种生化和细胞生物标志物的反应性（包括免疫参数，脂质和氧化代谢），遗传毒性作用的发生结合起来。化学分析显示，高碳酸血症对CBZ的积累和排泄的影响有限，而在基因表达谱和细胞水平的功能变化中观察到了复杂的生物反应网络。γ-氨基丁酸（GABA）途径的调制表明与脊椎动物已知的作用机理相似：免疫反应，细胞稳态和氧化系统代表着复合应激源的靶向过程。通过定量证据权重模型对结果进行整体阐述，