Biogenic volatile organic compounds (BVOCs) are metabolites emitted by living plants that have a fundamental ecological role since they influence atmospheric chemistry, plant communication and pollinator/herbivore behaviour, and human activities. Over the years, several strategies have been developed to isolate and identify them, and to take advantage of their activity. The main techniques used for in-vivo analyses include dynamic headspace (D-HS), static headspace (S-HS) and, more recently, direct contact (DC) methods in association with gas chromatography (GC) and mass spectrometry (MS). The aim of this review is to provide insight into the in-vivo characterisation of plant volatile emissions with a focus on sampling, analysis and possible applications. This review first provides a critical discussion of the challenges associated with conventional approaches and their limitations and advantages. Then, it describes a series of applications of in-vivo volatilomic studies to enhance how the information they provide impact on our knowledge of plant behaviour, including the effects of abiotic (damage, flooding, climate) and biotic (insect feeding) stress factors in relation to the plants.

中文翻译：

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植物挥发性排放物体内评价的分析策略——综述

生物挥发性有机化合物 (BVOC) 是活植物排放的代谢物，具有基本的生态作用，因为它们会影响大气化学、植物通讯和传粉媒介/食草动物行为以及人类活动。多年来，已经开发了几种策略来隔离和识别它们，并利用它们的活动。用于体内分析的主要技术包括动态顶空 (D-HS)、静态顶空 (S-HS) 以及最近与气相色谱 (GC) 和质谱 (MS) 相关的直接接触 (DC) 方法. 本综述的目的是深入了解植物挥发性排放物的体内表征，重点是采样、分析和可能的应用。本综述首先对与传统方法相关的挑战及其局限性和优势进行了批判性讨论。然后，它描述了体内挥发性研究的一系列应用，以增强它们提供的信息如何影响我们对植物行为的了解，包括非生物（损害、洪水、气候）和生物（昆虫喂养）胁迫因素对植物行为的影响。与植物的关系。