Copper and zinc have a high binding affinity with a Staphylococcus aureus bacterial community. This causes a change in the biomolecular composition of S. aureus. Our study aims at understanding the resistance mechanism of Cu and Zn either or in various combinations using FTIR and chemometric techniques. Zn toxicity resulted in a significant change in lipid content (3100-2800 cm-1) compared to Cu. A significant decrease in protein content is observed for Cu treatment in the amide region. The bio-concentration factor shows a higher value for Cu compared to Zn. The increase in band area of carbohydrates moieties 1059 cm-1 shows the secretion of EPS due to Cu toxicity. A significant change in nucleic acid compositions was noted in the region1200-900 cm-1 due to Zn treatment. Secondary structural change in protein shows β sheet formation. The result of the finding shows Cu has greater toxicity than Zn. Further toxicity effects were greatly enhanced for metal mixtures ratio (Cu:2Zn). This shows Zn exhibits synergism effect with Cu. The obtained ROC (receiver operating characteristic) curve area gives good reliability of the experiments. The study attempts to understand the mechanism of toxicity removal of Cu and Zn metal mixtures by bacterial population using FTIR coupled with chemometric techniques. Graphical abstract.

中文翻译：

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使用 FTIR 结合化学计量分析研究 Cu2+ 离子单独和与 Zn2+ 离子结合表征金黄色葡萄球菌分子变化的毒性机制

铜和锌与金黄色葡萄球菌细菌群落具有高结合亲和力。这会导致金黄色葡萄球菌的生物分子组成发生变化。我们的研究旨在使用 FTIR 和化学计量学技术了解 Cu 和 Zn 或以各种组合形式的电阻机制。与 Cu 相比，Zn 毒性导致脂质含量 (3100-2800 cm-1) 发生显着变化。在酰胺区域中观察到铜处理的蛋白质含量显着降低。与 Zn 相比，Cu 的生物浓度因子显示出更高的值。碳水化合物部分的带面积增加 1059 cm-1 表明由于 Cu 毒性导致 EPS 的分泌。由于锌处理，在 1200-900 cm-1 区域注意到核酸组成的显着变化。蛋白质的二级结构变化显示β折叠形成。研究结果表明，Cu 比 Zn 具有更大的毒性。金属混合物比例（Cu:2Zn）的进一步毒性作用大大增强。这表明Zn与Cu表现出协同效应。获得的 ROC（接收器操作特性）曲线区域提供了良好的实验可靠性。该研究尝试使用 FTIR 结合化学计量学技术了解细菌群对铜和锌金属混合物的毒性去除机制。图形概要。该研究尝试使用 FTIR 结合化学计量学技术了解细菌群对铜和锌金属混合物的毒性去除机制。图形概要。该研究尝试使用 FTIR 结合化学计量学技术了解细菌群对铜和锌金属混合物的毒性去除机制。图形概要。