We studied the effect of microvesicles derived from cells of the NK-92 cell line on the formation of tube-like structures by endothelial cells of the ЕА.Hy926 cell line. Microvesicles were isolated by differential centrifugation and their size was controlled by granulometric analysis using dynamic light scattering method. The effect of microvesicles produced by NK cells on angiogenesis was evaluated by cultural methods. In the course of the research, a model of co-culturing of microvesicles and endothelial cells on extracellular matrix Matrigel was developed. It was found that microvesicles derived from NK-92 cells promoted elongation of tube-like structures formed by endothelial ЕА.Hy926 cells. Microvesicles produced by NK cells can modulate functional activity of endothelial cells by affecting their ability to form blood vessels.

中文翻译：

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自然杀伤细胞产生的微泡调节血管的形成

我们研究了源自 NK-92 细胞系细胞的微泡对 ЕА.Hy926 细胞系内皮细胞形成管状结构的影响。通过差速离心分离微泡，并通过使用动态光散射方法的粒度分析控制它们的大小。通过培养方法评估NK细胞产生的微泡对血管生成的影响。在研究过程中，开发了微泡和内皮细胞在细胞外基质基质胶上共培养的模型。发现源自NK-92细胞的微泡促进由内皮ЕА.Hy926细胞形成的管状结构的伸长。NK细胞产生的微泡可以通过影响内皮细胞形成血管的能力来调节内皮细胞的功能活性。