The aim of this study was to evaluate the hepatoprotective effects of the fruits of Polygonum orientale L. (POE) against fibrosis in carbon tetrachloride (CCl₄)-induced liver injury. Bioactive components of POE were identified using liquid chromatography (LC)-mass spectrometry (MS)/MS by comparison with standards. Treatment with either silymarin (200 mg/kg) or POE (0.5 and 1.0 g/kg) caused significant decreases in the serum levels of enzymes and reduced the extent of liver lesions and fibrosis in histological analysis. POE (0.5 and 1.0 g/kg) decreased the levels of malondialdehyde, nitric oxide, proinflammatory cytokines (ie, tumor necrosis factor-α, interleukin [IL]-1β, and IL-6), an inflammatory cytokine (ie, cyclooxygenase-2), a profibrotic cytokine (ie, transforming growth factor-β), and fibrosis-related proteins (ie, connective tissue growth factor and α-smooth muscle actin) in the liver and enhanced the activities of the antioxidative enzymes superoxide dismutase, glutathione peroxidase, glutathione reductase, and catalase. Quantitative analysis of the active constituents in POE revealed an extract composition of 3.4 mg/g of protocatechuic acid, 20.8 mg/g of taxifolin, and 5.6 mg/g of quercetin. We have demonstrated that the hepatoprotective mechanisms of POE are likely to be associated with the decrease in inflammatory cytokines by increasing the activities of antioxidant enzymes. Our findings provide evidence that POE possesses a hepatoprotective activity to ameliorate chronic liver injury.

这项研究的目的是评估东方Poly（POE）果实对四氯化碳（CCl ₄）中纤维化的肝保护作用。）引起的肝损伤。通过与标准品比较，使用液相色谱（LC）-质谱（MS）/ MS鉴定POE的生物活性成分。水飞蓟素（200 mg / kg）或POE（0.5和1.0 g / kg）的治疗导致血清酶水平显着降低，并在组织学分析中减少了肝脏病变和纤维化的程度。POE（0.5和1.0 g / kg）降低了丙二醛，一氧化氮，促炎细胞因子（即肿瘤坏死因子-α，白介素[IL]-1β和IL-6），炎性细胞因子（即环加氧酶- 2），肝脏中的纤维化细胞因子（即转化生长因子-β）和纤维化相关蛋白（即结缔组织生长因子和α-平滑肌肌动蛋白），并增强了抗氧化酶超氧化物歧化酶，谷胱甘肽的活性过氧化物酶 谷胱甘肽还原酶和过氧化氢酶。对POE中活性成分的定量分析显示，提取物成分为3.4 mg / g原儿茶酸，20.8 mg / g滑石粉和5.6 mg / g槲皮素。我们已经证明，POE的肝保护机制可能与通过增加抗氧化酶的活性而降低炎症细胞因子有关。我们的发现提供了证据，证明POE具有减轻慢性肝损伤的保肝活性。我们已经证明，POE的肝保护机制可能与通过增加抗氧化酶的活性而降低炎症细胞因子有关。我们的发现提供了证据，证明POE具有减轻慢性肝损伤的保肝活性。我们已经证明，POE的肝保护机制可能与通过增加抗氧化酶的活性而降低炎症细胞因子有关。我们的发现提供了证据，证明POE具有减轻慢性肝损伤的保肝活性。