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Assessment of the microbial community and biocide resistance profile in production and injection waters from an Andean oil reservoir in Colombia
International Biodeterioration & Biodegradation ( IF 4.8 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.ibiod.2020.105137
Katherine Bedoya , Jhorman Niño , Julia Acero , Felipe Cabarcas , Juan F. Alzate

Abstract Water flooding is a widely applied method for secondary oil recovery. However, this practice introduces exogenous microorganisms into the oil reservoirs that can have deleterious consequences for the recovery process, such as hydrogen sulfide production and corrosion. Biocide treatments have been used to control harmful microbial activity. However, they tend to has limited efficacy attributed to the selection of resistant bacterial populations. This work combines Metagenomic and metataxonomic approaches to investigate the phylogenetic and functional profile of the produced and injected water from an oil reservoir located in the Andean region. The results reveal a marked dominance of the phylum Proteobacteria in both samples (nearly 99%). While Arcobacter sp. and Pseudomonas balearica were the dominant microbes in the injected water, Marinobacter sp. and Arcobacter sp. were the dominant bacteria in the produced water. Biocide resistance genes coding for efflux pumps and transporters were enriched in the injected water that is treated with a mixture of glutaraldehyde and THPS. The draft genome of the Pseudomonas balearica of the injection water encodes several proteins related to efflux pumps, while the Arcobacter sp. draft genome showed fewer proteins related to these resistance systems. Genome annotation of gene clusters related to secondary metabolism also showed that the Pseudomonas balearica present gene clusters for Amonabactin, Ectoine, and dipeptide N-acetyl glutaminyl glutamine amide (NAGNN), whereas the Arcobacter sp. possess one gene cluster for Bacteriocin.

中文翻译:

评估哥伦比亚安第斯油藏生产和注入水中的微生物群落和杀生物剂抗性概况

摘要 水驱是一种广泛应用的二次采油方法。然而,这种做法会将外源微生物引入油藏,这会对采收过程产生有害的后果,例如硫化氢的产生和腐蚀。杀菌剂处理已被用于控制有害的微生物活动。然而,由于选择了耐药菌群,它们往往具有有限的功效。这项工作结合了宏基因组学和元分类学方法,以研究来自安第斯地区油藏的采出水和注入水的系统发育和功能特征。结果表明,两种样品中变形菌门都具有显着优势(接近 99%)。虽然 Arcobacter sp。Pseudomonas balearica 是注入水中的优势微生物,海洋杆菌属 和弧菌属。是采出水中的优势细菌。编码外排泵和转运蛋白的杀生物剂抗性基因在用戊二醛和 THPS 的混合物处理的注入水中富集。注入水中的巴氏假单胞菌的基因组草图编码了几种与外排泵相关的蛋白质,而 Arcobacter sp. 基因组草图显示与这些抗性系统相关的蛋白质较少。与次生代谢相关的基因簇的基因组注释还表明,巴利阿里假单胞菌存在 Amonabactin、Ectoine 和二肽 N-乙酰谷氨酰胺酰谷氨酰胺 (NAGNN) 的基因簇,而 Arcobacter sp。拥有一个细菌素基因簇。编码外排泵和转运蛋白的杀生物剂抗性基因在用戊二醛和 THPS 的混合物处理的注入水中富集。注入水中的巴氏假单胞菌的基因组草图编码了几种与外排泵相关的蛋白质,而 Arcobacter sp. 基因组草图显示与这些抗性系统相关的蛋白质较少。与次生代谢相关的基因簇的基因组注释还表明,巴利阿里假单胞菌存在 Amonabactin、Ectoine 和二肽 N-乙酰谷氨酰胺酰谷氨酰胺 (NAGNN) 的基因簇,而 Arcobacter sp。拥有一个细菌素基因簇。编码外排泵和转运蛋白的杀生物剂抗性基因在用戊二醛和 THPS 的混合物处理的注入水中富集。注入水中的巴氏假单胞菌的基因组草图编码了几种与外排泵相关的蛋白质,而 Arcobacter sp. 基因组草图显示与这些抗性系统相关的蛋白质较少。与次生代谢相关的基因簇的基因组注释还表明,巴利阿里假单胞菌存在 Amonabactin、Ectoine 和二肽 N-乙酰谷氨酰胺酰谷氨酰胺 (NAGNN) 的基因簇,而 Arcobacter sp。拥有一个细菌素基因簇。注入水中的巴氏假单胞菌的基因组草图编码了几种与外排泵相关的蛋白质,而 Arcobacter sp. 基因组草图显示与这些抗性系统相关的蛋白质较少。与次生代谢相关的基因簇的基因组注释还表明,巴利阿里假单胞菌存在 Amonabactin、Ectoine 和二肽 N-乙酰谷氨酰胺酰谷氨酰胺 (NAGNN) 的基因簇,而 Arcobacter sp。拥有一个细菌素基因簇。注入水中的巴氏假单胞菌的基因组草图编码了几种与外排泵相关的蛋白质,而 Arcobacter sp. 基因组草图显示与这些抗性系统相关的蛋白质较少。与次生代谢相关的基因簇的基因组注释还表明,巴利阿里假单胞菌存在 Amonabactin、Ectoine 和二肽 N-乙酰谷氨酰胺酰谷氨酰胺 (NAGNN) 的基因簇,而 Arcobacter sp。拥有一个细菌素基因簇。和二肽 N-乙酰谷氨酰胺酰谷氨酰胺 (NAGNN),而 Arcobacter sp。拥有一个细菌素基因簇。和二肽 N-乙酰谷氨酰胺酰谷氨酰胺 (NAGNN),而 Arcobacter sp。拥有一个细菌素基因簇。
更新日期:2021-02-01
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