Bigels are materials with semi-solid organic and aqueous phases and, therefore, have the potential to deliver both lipophilic and hydrophilic compounds to humans. Additionally, because bigels are two-phase systems, a diverse array of synthesis options are possible, allowing many unique characteristics to be achieved. The purpose of this study was two-fold: to assess the ability of bigels to protect probiotics during in vitro digestion in a simulated gastrointestinal system and to determine the effect of phospholipids on probiotic survival. A standardized COST INFOGEST Network in vitro digestion system was used, and survival of Bifidobacterium lactis and Lactobacillus acidophilus was measured. By measuring the increase in free fatty acids, the enzymes’ lipolytic activity was confirmed. A control, with no gelators, had the greatest lipolysis. The formulated bigel was effective at protecting probiotics from the harsh conditions of the digestive tract compared to the control. L. acidophilus was more resistant to high shear conditions than B. lactis during gel preparation. Probiotics in bigels with phospholipids had greater survival during digestion; nonetheless, the difference was not statistically significant. It was hypothesized that this was due to the phospholipids being enzymatically broken down in the in vitro system. Therefore, for phospholipids to have a beneficial impact on probiotic survival, they must be in their native form. Overall, this study showed the potential of bigels to efficiently protect probiotics during digestion.

Bigels是具有半固态有机相和水相的材料，因此具有将亲脂性和亲水性化合物传递给人类的潜力。另外，由于双峰是两相系统，因此可能有各种各样的合成选项，从而可以实现许多独特的特性。这项研究的目的有两个：评估在模拟胃肠道系统中体外消化过程中双链蛋白保护益生菌的能力，并确定磷脂对益生菌存活的影响。使用标准化的COST INFOGEST网络体外消化系统，乳酸双歧杆菌和嗜酸乳杆菌的存活率被测量。通过测量游离脂肪酸的增加，证实了酶的脂解活性。没有胶凝剂的对照具有最大的脂解作用。与对照相比，配制的比格尔有效地保护了益生菌免受消化道的恶劣条件。在凝胶制备过程中，嗜酸乳杆菌比乳酸杆菌更能抵抗高剪切条件。含磷脂的双糖中的益生菌在消化过程中具有更大的存活率；但是，差异在统计上并不显着。假设这是由于磷脂在体外被酶促分解系统。因此，为了使磷脂对益生菌的存活产生有益的影响，它们必须为天然形式。总体而言，这项研究表明，双链菌具有在消化过程中有效保护益生菌的潜力。