Fenton-like reaction-associated chemodynamic therapy (CDT) and hyperthermia-inducing photothermal therapy (PTT)-combined crosslinked hydrogel systems were developed for loco-regional cancer therapy. Cupric sulfate (Cu) has been employed to crosslink the catechol-functionalized hyaluronic acid (HC) polymer-based gel via metal–catechol coordination and covalent bonding of the catechol group (by pH adjustment). Cu can also be used as a hydroxyl radical-generating agent with endogenous H₂O₂ in cancer cells mediated by Fenton-like reaction and it can reduce intracellular glutathione (GSH) levels leading to the inhibition of reactive oxygen species (ROS) scavenging. These two strategies can amplify the ROS-initiated CDT efficiency for combating cancer. The Cu-incorporated crosslinked hydrogel structure with pH modulation was appropriate for injectable gel formation via a single syringe. The incorporation of indocyanine green (ICG) into the hydrogel network and near-infrared (NIR) laser irradiation provided a temperature elevation sufficient for induction of hyperthermia in cancer therapy. It is expected that the designed HC/Cu/ICG hydrogel can be used safely and efficiently for local CDT and PTT of breast cancer.

中文翻译：

---

硫酸铜交联的Fenton样反应，谷胱甘肽还原和光热消融内置水凝胶用于局部区域癌症治疗

Fenton样反应相关的化学动力学疗法（CDT）和高热诱导光热疗法（PTT）结合的交联水凝胶系统被开发用于局部区域癌症治疗。硫酸铜（Cu）已用于通过金属-邻苯二酚配位和邻苯二酚基团的共价键合（通过pH调节）使基于邻苯二酚官能化的透明质酸（HC）聚合物交联。Cu还可以用作具有内源性H ₂ O ₂的羟基自由基产生剂在Fenton样反应介导的癌细胞中，它可以降低细胞内谷胱甘肽（GSH）的水平，从而抑制活性氧（ROS）清除。这两种策略可以增强ROS引发的CDT对抗癌症的效率。具有pH调节功能的掺有Cu的交联水凝胶结构适合通过单个注射器形成可注射的凝胶。将吲哚菁绿（ICG）掺入水凝胶网络和近红外（NIR）激光照射中，可提供足以在癌症治疗中诱导高热的温度升高。预期设计的HC / Cu / ICG水凝胶可安全有效地用于乳腺癌的局部CDT和PTT。