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In vitro and in vivo antifungal activity of Cuminum cyminum essential oil against Aspergillus aculeatus causing bunch rot of postharvest grapes
PLOS ONE ( IF 3.7 ) Pub Date : 2020-11-24 , DOI: 10.1371/journal.pone.0242862
Chutima Tanapichatsakul , Sarunpron Khruengsai , Patcharee Pripdeevech

Bunch rot in grapes is an aggressive disease and needs to be controlled during the postharvest period. We investigate the antifungal potential of Zanthoxylum bungeanum Maxim., Zanthoxylum rhetsa, Cuminum cyminum, Coriandrum sativum, and Zingiber montanum (J. Koenig) Link ex A. Dietr. essential oils against Aspergillus aculeatus that cause bunch rot disease on postharvest grapes. C. cyminum essential oil exhibited stronger significantly inhibition percentage of 95.08% than other treatments in in vitro assay. Cumin aldehyde (33.94%) and α-terpinen-7-al (32.20%) were identified as major volatile compounds in C. cyminum oil. Antifungal potential of C. cyminum oil was then tested in conidia germination and in vitro tests compared to cumin aldehyde and α-terpinen-7-al. Their EC50 values against the conidial germination were also estimated. Significant reduction of conidia germination was also detected in C. cyminum essential oil and cumin aldehyde at a concentration of 1,000 and 100 μg/mL, respectively. EC50 values of the C. cyminum essential oil, cumin aldehyde, and α-terpinen-7-al were 67.28 μg/mL, 9.31 μg/mL, and 13.23 μg/mL, respectively. In vivo assay, the decrease of the disease severity (0.69%) and incidence (1.48%) percentage of A. aculeatus on grape berries treated at 1,000 μg/mL of C. cyminum essential oil was significantly greater than that obtained from other treatments after 10 days incubation. In addition, grape berries treated with C. cyminum essential oil decreased weight loss and retained fruit firmness. The changing of total soluble solids, total phenolic content, and antioxidant activity are also delayed in treated fruits. Therefore, essential oil of C. cyminum may be applied as a biological antifungal agent to control A. aculeatus in postharvest grapes without any negative effects on its quality.



中文翻译:

孜然精油对引起采后葡萄串腐的曲霉的体外体内抗真菌活性。

葡萄中的串腐是一种侵害性疾病,需要在采后阶段加以控制。我们调查的抗真菌潜力花椒马克西姆,花椒rhetsa孜然芫荽生姜montanum(J.凯尼格)链接前A. Dietr。抗尖孢曲霉的精油,可引起采后葡萄的串腐病。Ç精油中表现出比其它处理更强显著百分比的抑制95.08%的体外测定法。小茴香醛(33.94%)和α-萜品7-al(32.20%)被确定为主要的挥发性化合物Ç芹菜油。C的抗真菌潜力。然后,与小茴香醛和α-萜品7a-al相比,在子孢子萌发和体外试验中测试了次生植物精油的含量。还估计了其针对分生孢子萌发的EC 50值。在C中还检测到分生孢子萌发明显减少。cyminum精油和小茴香醛的浓度分别为1,000和100μg/ mL。C的EC 50值。cyminum精油,孜然醛和α-萜品7-al分别为67.28μg/ mL,9.31μg/ mL和13.23μg/ mL。体内检测,疾病严重程度的降低(0.69%)和发生率(1.48%)的A百分比。以1,000μg / mL的C处理的葡萄浆果上的刺突。孵育10天后,Cyminum精油显着大于从其他处理中获得的精油。此外,用C处理过的葡萄浆果。cyminum精油减少了体重减轻并保留了水果的硬度。经处理的水果中总可溶性固形物,总酚含量和抗氧化活性的变化也被延迟。因此,C的精油。孜然可以以控制的生物抗真菌剂被施加收获后的葡萄中的刺孔雀科对其品质没有任何负面影响。

更新日期:2020-11-25
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