Abstract

Azo dyes constitute a significant environmental burden due to its toxicity, carcinogenicity, and hard biodegradation. The report here is focused on the decolorization and degradation treatment of azo dye methyl red (MR). Decolorization of MR using Aspergillus versicolor LH1 isolated from activated sludge was investigated. The maximum decolorization rate of 92.3% was obtained under the optimized conditions of sucrose as carbon source, 5d incubation age, pH 6.0, 140 mg/L initial concentration of MR and 2.5 g/L initial concentration of NaNO₃. Biodegradation products of MR were investigated using HPLC-MS, FTIR, and GC-MS assays. It was revealed the three bonds of –C–N = in MR aromatic nucleus were disrupted, and benzoic acid was detected. Micronucleus test with Glycine max L. and Vicia faba L. demonstrated that MCN‰ (micronucleus permillage) of MR metabolites was less than MR solution. These findings provided evidence that A. versicolor LH1 is a candidate for MR degradation in industrial wastewater treatment.

摘要

由于其毒性、致癌性和难以生物降解，偶氮染料构成了重大的环境负担。这里的报告重点是偶氮染料甲基红（MR）的脱色和降解处理。研究了使用从活性污泥中分离的花曲霉LH1对 MR 的脱色。在以蔗糖为碳源、培养5d、pH 6.0、MR初始浓度140 mg/L和NaNO ₃初始浓度2.5 g/L的优化条件下，脱色率最高可达92.3% 。使用 HPLC-MS、FTIR 和 GC-MS 分析研究了 MR 的生物降解产物。发现MR芳核中-C-N=的三个键被破坏，并检测到苯甲酸。使用Glycine max 进行微核测试L. 和蚕豆L. 证明 MR 代谢物的 MCN‰（微核 permillage）小于 MR 溶液。这些发现提供了证据，表明A. versicolor LH1 是工业废水处理中 MR 降解的候选物。