We accessed the effects of dietary Zn on growth, immunity and reproductive performance in female Macrobrachium nipponense. After feeding with a homemade diet containing 21, 50, 81, 136 and 203 mg kg⁻¹ Zn for 60 days, prawns showed a significant increase in weight gain, improved survival, higher hepatosomatic and gonadosomatic indices (p < 0.05) in group 50 and 81 mg kg⁻¹ Zn. Broken‐line analysis indicated that the optimal concentration of dietary zinc was 75.48 mg kg⁻¹. Dietary Zn had drastically increased crude lipid content in body composition (p < 0.05), while moisture, ash and crude protein had no significant difference among groups (p > 0.05). Zn content in hepatopancreas linearly increased along with increases in dietary Zn, while Zn content in muscle and haemolymph had no difference in Zn supplemented groups (p > 0.05). The highest activities of Cu/Zn‐SOD, T‐SOD and CAT in hepatopancreas were in 81 mg kg⁻¹ and 50 mg kg⁻¹ Zn diet, respectively. MDA had the lowest content in 21 to 81 mg kg⁻¹ Zn levels. The highest haemolymph AKP activity was in 81 mg kg⁻¹ Zn diet group, while the highest PO activity was in 136 mg kg⁻¹ Zn diet. Both levels of progesterone and estradiol and gene expression of VG and VGR were highest in 50 to 81 mg kg⁻¹ Zn levels. These results provided evidence that dietary Zn ranging from 50 to 81 mg kg⁻¹ has a positive impact on growth, immunity and reproductive performance of female prawn.

中文翻译：

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日粮锌对雌性日本沼虾生长，免疫和生殖性能的影响

我们获得了饮食锌对雌性日本沼虾生长，免疫和生殖性能的影响。在第21组，对虾饲喂含有21、50、81、136和203 mg kg ^-1 Zn的自制饮食60天后，对虾显示出体重增加显着增加，存活率提高，肝体和性体激素指数更高（p  <0.05）和81 mg kg ^-1 Zn。折线分析表明，膳食锌的最佳浓度为75.48 mg kg ^-1。饮食中锌使人体组成中的粗脂肪含量急剧增加（p  <0.05），而水分，灰分和粗蛋白在各组之间无显着性差异（p > 0.05）。肝胰腺中的锌含量随膳食中锌的增加而线性增加，而肌肉和血淋巴中的锌含量在锌补充组中无差异（p  > 0.05）。肝胰腺中Cu / Zn-SOD，T-SOD和CAT的最高活性分别为81 mg kg ^-1和50 mg kg ^-1锌饮食。MDA含量最低，为21至81 mg kg ^-1 Zn。血淋巴AKP活性最高的是81 mg kg ^-1锌饮食组，而最高的PO活性是136 mg kg ^-1 Zn饮食组。孕酮和雌二醇的水平以及VG和VGR的基因表达在50至81 mg kg ^-1中均最高锌水平。这些结果提供了饮食锌在50至81 mg kg ^-1范围内对雌虾的生长，免疫和生殖性能具有积极影响。