Abstract In vitro propagation can be explored to overcome the constraint of limited planting material in plum. Although it ensures the production of plants in large number, but there are chances of somaclonal variations using this technology. Thus, clonal fidelity of in vitro raised plants should be checked to obtain true to type planting material prior to transplantation in the field. Genetic fidelity of in vitro cultures of plum (Prunus salicina) cvs. Santa Rosa and Frontier multiplied for 5 years (60 passages) through enhanced axillary bud proliferation was tested and compared with 2 year old in vitro raised and mother plants of respective cultivars using RAPD, ISSR and SCoT markers. Out of twenty-eight RAPD primers, eighteen produced 29 and 27 distinct bands in Santa Rosa and Frontier whereas, all of the fifteen ISSR primers screened generated clear reproducible bands in both the cultivars. In SCoT assay, eight primers out of twenty-six generated reproducible bands in both the cultivars. Homogenous amplification was observed in all the samples thereby confirming the genetic fidelity of tissue culture raised plants, thus suggesting that in vitro propagation using axillary buds is the safest mode for the production of clonal planting material in plum.

中文翻译：

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通过 RAPD、ISSR 和 SCoT 标记对日本李子 cvs Santa Rosa 和 Frontier 中长期体外芽培养和再生植物的遗传保真度评估

摘要 可以探索离体繁殖，以克服李子种植材料有限的限制。虽然它确保了大量植物的生产，但使用该技术存在体细胞克隆变异的机会。因此，应在田间移植前检查体外培育植物的克隆保真度，以获得与类型种植材料相符的材料。李子 (Prunus salicina) cvs 体外培养物的遗传保真度。Santa Rosa 和 Frontier 通过增强的腋芽增殖繁殖了 5 年（60 次传代），并使用 RAPD、ISSR 和 SCoT 标记与 2 岁的体外培育和各自栽培品种的母株进行比较。在 28 个 RAPD 引物中，有 18 个在 Santa Rosa 和 Frontier 产生了 29 和 27 个不同的条带，而，筛选的所有 15 种 ISSR 引物都在两个品种中产生了清晰的可重复条带。在 SCoT 测定中，26 个引物中的 8 个引物在两个品种中产生了可重现的条带。在所有样品中均观察到同质扩增，从而证实了组织培养培育植物的遗传保真度，从而表明使用腋芽的体外繁殖是生产李子克隆种植材料的最安全模式。