Aedes aegypti is one of the vectors responsible for transmitting the viruses that cause dengue, Zika and chikungunya in the human population. Mosquitoes have bacterial communities in different organs, mainly in the midgut, but to a lesser extent in their reproductive organs, such as the ovaries, where replication and vertical transmission is decisive for dengue virus. These bacteria also influence metabolic and physiological processes such as ingestion and digestion of blood. In this study, aerobic bacterial communities associated with ovaries of A. aegypti Rockefeller strain were determined, describing their potential function during ovocitary development. The groups of mosquitoes were separated into three treatments: diet with 10% sugar solution, diet with blood supply, and blood feeding combined with tetracycline. The ovaries were extracted from the mosquitoes, and then put in enriched culture media (blood and nutritive agar) by direct inoculation, for subsequent isolation and macroscopic and microscopic characterization of the colonies. The taxonomic determination of bacterial isolates was achieved by sequence analysis of the 16S rRNA gene. A higher bacterial load was observed in the sugar feeding group (6 × 10³ CFU/ml) in contrast to the group fed only with blood, with and without an antibiotic (4.03-4.04 × 10³CFU/ml; 4.85-5.04 × 10³CFU/ml). As a result, a total of 35 colonies were isolated, of which 80% were gram-negative and 20% gram-positive; 72% were lactose negative and 8% lactose positive. Of the total bacteria, 83% had gamma hemolysis, 17% alpha hemolysis, and none presented beta hemolysis. After phenotypic and biochemical characterization, 17 isolates were selected for molecular identification. Only phyla Actinobacteria and Proteobacteria were found. Bacteria associated with ovaries of A. aegypti were mainly identified as belonging to the Serratia and Klebsiella genera. Some bacteria (Serratia marcescens, Pantoea dispersa and Klebsiella oxytoca) have wide biotechnological potential due to their entomopathogenic power and their bioactivity against different pathogens.

埃及伊蚊是负责在人类中传播引起登革热，寨卡病毒和基孔肯雅热的病毒的载体之一。蚊子在不同器官中都有细菌群落，主要在中肠，但在生殖器官（如卵巢）中的程度较小，那里的复制和垂直传播对登革热病毒起决定性作用。这些细菌还会影响代谢和生理过程，例如血液的摄入和消化。在这项研究中，有氧细菌群落与埃及伊蚊的卵巢有关确定了洛克菲勒应变，描述了它们在卵子发育过程中的潜在功能。蚊子分为三种处理：含10％糖溶液的饮食，有血液供应的饮食，以及与四环素结合的血液喂养。从蚊子中提取卵巢，然后通过直接接种将其置于丰富的培养基（血液和营养琼脂）中，以用于随后的分离以及菌落的宏观和微观表征。细菌分离株的分类学测定是通过对16S rRNA基因进行序列分析来实现的。与仅喂食有血和不喂抗生素的组（4.03-4.04×10³CFU/ ml； 4.85-5.04×10³CFU/ ml）相比，糖喂食组（6×10³CFU / ml）的细菌载量更高。 ）。结果，总共分离出35个菌落，其中80％为革兰氏阴性和20％革兰氏阳性；72％为乳糖阴性，而8％为乳糖阳性。在全部细菌中，有83％发生了伽玛溶血，17％发生了α溶血，没有细菌发生过β溶血。在表型和生化特性鉴定后，选择了17种分离物进行分子鉴定。仅发现门放线菌和变形杆菌。与卵巢相关的细菌埃及伊蚊主要被鉴定为属于沙雷氏菌和克雷伯菌属。一些细菌（粘质沙雷氏菌（Serratia marcescens），泛菌（Pantoea dispersa）和产酸克雷伯氏菌（Klebsiella oxytoca））由于其昆虫致病力和对不同病原体的生物活性而具有广泛的生物技术潜力。