Anther culture is the most effective tool for doubled haploid production of wheat. This investigation was conducted to estimate genetic parameters of anther culture response in wheat and identification of putative Quantitative trait loci (QTLs) associated with response traits. Two varieties of wheat, namely ICR-DH (P₁) and Sle 1 × 15 (P₂) and their F₁ and F₂ progenies were used in the present investigation to estimate genetic parameters of anther culture response. Two molecular marker systems, SRAP and SSR markers were used to detect the polymorphism between two anther donor parents. Single marker analysis (SMA) and Composite interval mapping (CIM) were used to localize the putative QTL associated with four anther culture response in wheat using 100 plants of F₂ population derived from F₁ cross ‘ICR-DH’ × ‘Sel 1 × 15’. Analyses of variance indicated significant differences between four populations (P₁, P₂, F₁ and F₂) for callus induction (CAL), number of green plants per 100 anther (GR), number of albino plant per 100 anther (AR) and total regenerated plants per 100 anther (TR). The additive effects were more important than dominance effects in controlling these traits. The two molecular marker systems were sufficient in detecting polymorphism between two parents. Thirty two putative QTLs were detected on eight linkage groups. Our study indicated that the additive effects of genes and detection of new QTLs permit marker-assisted selection of genotypes with high green plantlet regeneration efficiency in anther culture, and therefore favor efficient use of anther culture in wheat breeding programs

Anther culture is the most effective tool for doubled haploid production of wheat. This investigation was conducted to estimate genetic parameters of anther culture response in wheat and identification of putative Quantitative trait loci (QTLs) associated with response traits. Two varieties of wheat, namely ICR-DH (P₁) and Sle 1 × 15 (P₂) and their F₁ and F₂ progenies were used in the present investigation to estimate genetic parameters of anther culture response. Two molecular marker systems, SRAP and SSR markers were used to detect the polymorphism between two anther donor parents. Single marker analysis (SMA) and Composite interval mapping (CIM) were used to localize the putative QTL associated with four anther culture response in wheat using 100 plants of F₂ population derived from F₁ cross ‘ICR-DH’ × ‘Sel 1 × 15’. Analyses of variance indicated significant differences between four populations (P₁, P₂, F₁ and F₂）中的愈伤组织诱导（CAL），每100颗花药的绿色植物数量（GR），每100颗花药的白化植物数量（AR）和每100颗花药的再生植物总数（TR）。在控制这些性状上，加性效应比优势效应更重要。两个分子标记系统足以检测两个亲本之间的多态性。在八个连锁组上检测到32个假定的QTL。我们的研究表明，基因的累加效应和新QTL的检测允许在花药培养中利用标记辅助选择具有高绿色小植株再生效率的基因型，因此有利于在小麦育种计划中有效利用花药培养