MicroRNA (miRNA) profiles were studied in roots of ‘Sanhu’ tangerine plants with and without Candidatus Liberibacter asiaticus (CLas) infections by using solexa sequencing to investigate the possible involvement of miRNAs in the pathogenesis of Citrus Greening (Huanglongbing) disease. A total of 186 known miRNAs and 71 novel miRNAs were thus identified. Of them, 37 known and 18 novel miRNAs were significantly different in their expression patterns in roots of CLas-free and CLas-infected plants. CLas infection induced de novo expression of 6 known miRNAs and up-regulated the expression of 13 known miRNAs. It also prevented the expression of 11 known miRNAs and down-regulated the expression of 7 others. A total of 316 genes were predicted to be the targets of these differentially expressed miRNAs. Particularly, 34, 28, 21, and 20 genes were predicted to be the respective targets of cre-miR156a, cre-miR396b, cre-miR396g-5p, and cre-miRn70. The predicted putative target genes are known to be involved in stress response, plant growth and development, transcription, and metabolism. Quantitative analyses of the expression of several representative miRNAs and their target genes were generally in agreement with the predicted expression patterns (inverse proportion). Further research to unravel the interactions between these miRNAs and their target genes and determine the associated biological and pathological significance is recommended.

微RNA（miRNA）谱是在“三湖”橘的植物有和无根的研究暂定Liberibacter胭脂（ç使用SOLEXA测序来研究miRNA在柑橘黄龙（黄龙）病的发病机制可能参与LAS）感染。因此，总共鉴定出186种已知的miRNA和71种新颖的miRNA。其中，37倍已知的，并且18新的miRNA是在根及其表达模式显著不同Ç拉斯-自由和Ç LAS-感染的植物。CLas感染可诱导6种已知miRNA的从头表达，并上调13种已知miRNA的表达。它还阻止了11种已知miRNA的表达，并下调了7种其他miRNA的表达。预计共有316个基因是这些差异表达的miRNA的靶标。特别是，预测有34、28、21和20个基因分别是cre-miR156a，cre-miR396b，cre-miR396g-5p和cre-miRn70的靶标。已知预测的推定靶基因与胁迫反应，植物生长发育，转录和代谢有关。几种代表性miRNA及其靶基因表达的定量分析通常与预测的表达模式一致（反比例）。