Cytoplasmic male sterility (CMS) phenomenon is widely exploited in commercial hybrid seed production in economically important crop species, including rye, wheat, maize, rice, sorghum, cotton, sugar beets, and many vegetables. Although some commercial successes, little is known about QTLs responsible for the trait in case of triticale with sterilizing Triticum timopheevii (Tt) cytoplasm. Recombinant inbred line (RIL) F6 mapping population encompassing 182 individuals derived from the cross of individual plants representing the HT352 line and cv Borwo was employed for genetic map construction using SNP markers and identification of QTLs conferring pollen sterility in triticale with CMS Tt. The phenotypes of the F1 lines resulting from crossing of the HT352 (Tt) with HT352 (maintainer) × Borwo were determined by assessing the number of the F2 seeds per spike. A genetic map with 21 linkage groups encompasses 29,737 markers and spanned over the distance of 2549 cM. Composite (CIM) and multiple (MIM) interval mappings delivered comparable results. Single QTLs mapped to the 1A, 1B, 2A, 2R, 3B, 3R, 4B, and 5B chromosomes, whereas the 5R and 6B chromosomes shared 3 and 2 QTLs, respectively. The QTLs with the highest LOD score mapped to the 5R, 3R, 1B, and 4B chromosomes; however, the QRft-5R.3 has the highest explained variance of the trait.

细胞质雄性不育 (CMS) 现象在经济上重要的作物物种的商业杂交种子生产中被广泛利用，包括黑麦、小麦、玉米、水稻、高粱、棉花、甜菜和许多蔬菜。尽管取得了一些商业上的成功，但对于小黑麦与Triticum timopheevii 的灭菌有关的 QTL知之甚少(Tt) 细胞质。重组自交系 (RIL) F6 作图群体包含来自代表 HT352 系和 cv Borwo 的单株杂交的 182 个个体，用于使用 SNP 标记构建遗传图谱，并使用 CMS Tt 鉴定赋予小黑麦花粉不育性的 QTL。通过评估每个穗的 F2 种子数来确定由 HT352 (Tt) 与 HT352 (维护者) × Borwo 杂交产生的 F1 品系的表型。具有 21 个连锁群的遗传图谱包含 29,737 个标记，跨越 2549 cM 的距离。复合 (CIM) 和多重 (MIM) 间隔映射提供了可比较的结果。单个 QTL 定位到 1A、1B、2A、2R、3B、3R、4B 和 5B 染色体，而 5R 和 6B 染色体分别共享 3 个和 2 个 QTL。LOD得分最高的QTL定位于5R、3R、1B和4B染色体；然而，QRft-5R.3 具有最高的性状解释方差。