A polysafranin/multiwall carbon nanotube-Nafion hybrid film was prepared by electropolymerizing safranin on the surface of a MWCNT-Naf/CPE. The modified electrode was evaluated as an electrochemical sensor for hydrogen peroxide (H₂O₂) and Cu²⁺ in the presence of H₂O₂, in 0.1 M Tris buffer (pH 6.0) using cyclic and differential pulse voltammetric methods. The electrode showed a pair of well-defined and quasi-reversible redox peaks with formal potential (E°΄) = 0.290 ± 0.005 V versus Ag/AgCl corresponding to the polysafranin redox couples. The effects of the experimental variables on the electrode behavior regarding polymerization conditions, electrolyte pH, and MWCNT concentration were investigated to obtain optimum experimental conditions. The investigation of differential pulse voltammetric results showed that the polysafranin film at the modified electrode surface acts as an effective catalyst for H₂O₂ r and Cu²⁺ reduction in the presence of H₂O₂. Under optimum conditions, the modified electrode exhibited a linear dynamic range of 4.5–23.0 mM and a sensitivity of 17.4 ± 0.4 μA mM^-1 cm^‑2 toward H₂O₂ with a detection limit of 0.6 mM (3S_bl/m). In the presence of 17.0 mM H₂O₂, the electrode showed a linear response range of 1.0–80.0 μM and a sensitivity of 7.6 ± 0.2 μA μM^‑1 cm^‑2, with a detection limit of 0.1 μM (3S_bl/m) for Cu²⁺. In the next step, using differential pulse voltammetry, the response of the electrode to Cu²⁺ was studied in the presence of interfering metal ions that can promote Fenton’s reaction. The results showed that Fe²⁺, Co²⁺, and Al³⁺ produce interference at more than 5-, 2- and 10-fold ratios ([cation]/[Cu²⁺]) respectively.

藏红素/多壁碳纳米管-Nafion杂化膜是通过将藏红素在MWCNT-Naf / CPE表面上电聚合而制得的。使用循环和微分脉冲伏安法，在0.1 M Tris缓冲液（pH 6.0）中，在H ₂ O ₂存在下，将改性电极作为过氧化氢（H ₂ O ₂）和Cu ²⁺的电化学传感器进行评估。电极显示出一对定义良好且准可逆的氧化还原峰，具有形式电位（E相对于对应于聚番红花素氧化还原对的Ag / AgCl，为0.29±0.005V。研究了有关聚合条件，电解质pH和MWCNT浓度的实验变量对电极行为的影响，以获得最佳实验条件。对差分脉冲伏安法结果的研究表明，在存在H ₂ O ₂的情况下，修饰电极表面的番红花膜可作为H ₂ O ₂ r和Cu ²⁺还原的有效催化剂。在最佳条件下，修饰电极的线性动态范围为4.5–23.0 mM，对H的灵敏度为17.4±0.4μAmM ^-1  cm ^-2检测限为0.6 mM（3S _bl / m）的₂ O ₂ 。在17.0毫摩尔H的存在₂ Ó ₂，电极显示出1.0-80.0微米的线性响应范围和7.6±0.2μAμM的灵敏度^-1 厘米^-2，0的检测极限0.1  μM（3S _BL / m）对于Cu ²⁺。在下一步中，使用差分脉冲伏安法，在存在可以促进Fenton反应的干扰金属离子的情况下，研究了电极对Cu ²⁺的响应。结果表明，Fe 2 ⁺，Co ²⁺和Al ³⁺分别以超过5倍，2倍和10倍的比率（[阳离子] / [Cu ²⁺ ]）产生干扰。