The study was designed to create a primary cell culture of uveal melanoma and to evaluate its resistance to chemotherapy. Of the obtained 20 samples of uveal melanoma, the primary cultures with proliferation sufficient for MTT test were derived in only 7 cases. However, even these cultures were unable to survive more than 4 passages; the cells accumulated melanin and underwent apoptosis. Retinol palmitate and nepafenac produced no cytotoxic effect on uveal melanoma cells. Of 5 cultures treated with sodium valproate (Convulex), no pronounced cytotoxic effect was observed in one culture (UM4); in 2 cultures, 50% cells died in the presence of the lowest drug concentration of 1.88 mg/ml; and in 2 cultures, the same effect was achieved at drug concentrations 7-10 mg/ml. The cytotoxic effect of treosulfan was evaluated in only 4 cultures of uveal melanoma: the drug exhibited pronounced antitumor activity on all cultures, in 2 cases, it was effective at a concentration of 0.16 mg/ml. Gemcitabine in a concentration of 2.5 mg/ml produced a pronounced cytotoxic effect in 4 out of 7 cultures (death of 70-80% cells) and induced death of ~45% cells in the remaining 3 cultures. Mitoxantrone had ambiguous effect: in 2 of 5 cultures, the drug in high concentrations stimulated the growth of tumor cells, but in 3 cultures, the drug even in minimum concentrations induced death of 70-80% cells.

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体外葡萄膜黑色素瘤细胞的化学敏感性评估

该研究旨在创建葡萄膜黑色素瘤的原代细胞培养物并评估其对化疗的抵抗力。在获得的20个葡萄膜黑色素瘤样本中，只有7个病例获得了足以进行MTT测试的增殖的原代培养物。然而，即使是这些培养物也无法存活超过 4 代；细胞积累黑色素并经历细胞凋亡。视黄醇棕榈酸酯和奈帕芬酸对葡萄膜黑色素瘤细胞没有细胞毒性作用。在用丙戊酸钠 (Convul​​ex) 处理的 5 种培养物中，一种培养物 (UM4) 未观察到明显的细胞毒性作用；在 2 个培养物中，50% 的细胞在最低药物浓度 1.88 mg/ml 存在下死亡；并且在 2 个培养物中，药物浓度为 7-10 mg/ml 时获得了相同的效果。仅在 4 种葡萄膜黑色素瘤培养物中评估了硫丹的细胞毒作用：该药物对所有培养物均表现出明显的抗肿瘤活性，在 2 例中，它在 0.16 mg/ml 的浓度下有效。浓度为 2.5 mg/ml 的吉西他滨在 7 种培养物中的 4 种中产生明显的细胞毒性作用（70-80% 细胞死亡），并在其余 3 种培养物中诱导约 45% 细胞死亡。米托蒽醌的作用不明确：在 5 个培养物中的 2 个中，高浓度的药物刺激了肿瘤细胞的生长，但在 3 个培养物中，即使是最低浓度的药物也诱导了 70-80% 的细胞死亡。5mg/ml 在 7 个培养物中的 4 个中产生明显的细胞毒性作用（70-80% 细胞死亡），并在其余 3 个培养物中诱导约 45% 细胞死亡。米托蒽醌的作用不明确：在 5 个培养物中的 2 个中，高浓度药物刺激了肿瘤细胞的生长，但在 3 个培养物中，即使是最低浓度的药物也诱导了 70-80% 的细胞死亡。5mg/ml 在 7 个培养物中的 4 个中产生明显的细胞毒性作用（70-80% 细胞死亡），并在其余 3 个培养物中诱导约 45% 细胞死亡。米托蒽醌的作用不明确：在 5 个培养物中的 2 个中，高浓度药物刺激了肿瘤细胞的生长，但在 3 个培养物中，即使是最低浓度的药物也诱导了 70-80% 的细胞死亡。