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A simple method of drying polyacrylamide slab gels that eliminates cracking.
Biotechniques ( IF 2.7 ) Pub Date : 2020-11-23 , DOI: 10.2144/btn-2020-0117
Ghanshyam D Heda 1
Affiliation  

The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable their detection and identification. Dried protein gels can also serve as an ideal method of preserving the gel itself for permanent record-keeping and allowing densitometry at a convenient time. Here I describe a simple and highly reproducible gel-drying method that results in dried gels without the cracks that are frequently encountered with many existing gel-drying methods.

中文翻译:

一种干燥聚丙烯酰胺平板凝胶的简单方法,可消除开裂。

聚丙烯酰胺平板凝胶是最常见的凝胶形式,用于通过电泳分析蛋白质样品。干燥这些凝胶可用于许多生物学应用。例如,放射自显影,其中放射性标记的蛋白质被分离以使其能够检测和识别。干燥的蛋白质凝胶也可以作为一种理想的方法来保存凝胶本身以永久保存记录并允许在方便的时间进行密度测定。在这里,我描述了一种简单且高度可重复的凝胶干燥方法,该方法可以使干燥的凝胶没有许多现有凝胶干燥方法经常遇到的裂缝。
更新日期:2020-11-25
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