Survivin is an inhibitor of apoptosis as well as a promoter of cell proliferation. Fibulin-3 is a matrix glycoprotein that displays potential for tumor suppression or propagation. This study aimed to validate the expression levels of survivin and fibulin-3 in benign and malignant respiratory diseases. This case-control study included 219 patients categorized into five groups. Group A included 63 patients with lung cancer, group B included 63 patients with various benign lung diseases, group D included 45 patients with malignant pleural mesothelioma (MPM), and group E included 48 patients with various benign pleural diseases. Group C included 60 healthy individuals (control group). Serum survivin and fibulin-3 levels were measured by ELISA, whereas their nuclear expressions in the lung and pleura were assessed via Western blot analysis. The results showed significantly higher survivin serum levels and significantly lower fibulin-3 levels in group A compared with in group B and controls (p<0.001). There were significantly higher serum levels of survivin and fibulin-3 in group D compared with in group E and controls (p<0.001), consistent with observed nuclear survivin and fibulin-3 expression levels. Fibulin-3 was determined to have higher value than survivin in discriminating lung cancer from MPM (p˂0.05). Survivin and fibulin-3 could be useful diagnostic markers for lung and pleural cancers, and fibulin-3 expression was particularly useful in differentiating lung cancer from MPM. Trial registration: ClinicalTrials.gov Identifier: NCT04413292: https://clinicaltrials.gov/ct2/show/NCT04413292, retrospectively registered.

中文翻译：

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Survivin和fibulin-3基因的循环和局部核表达在区分良恶性呼吸系统疾病中的相关性分析。

Survivin 是细胞凋亡的抑制剂，也是细胞增殖的促进剂。Fibulin-3 是一种基质糖蛋白，具有抑制或增殖肿瘤的潜力。本研究旨在验证 survivin 和 fibulin-3 在良恶性呼吸道疾病中的表达水平。该病例对照研究包括 219 名患者，分为五组。A组肺癌63例，B组各种良性肺部疾病63例，D组恶性胸膜间皮瘤（MPM）45例，E组各种良性胸膜疾病48例。C组包括60名健康个体（对照组）。通过ELISA测量血清存活蛋白和fibulin-3水平，而通过蛋白质印迹分析评估它们在肺和胸膜中的核表达。结果显示，与 B 组和对照组相比，A 组的 survivin 血清水平显着升高，fibulin-3 水平显着降低（p<0.001）。与 E 组和对照组相比，D 组中 survivin 和 fibulin-3 的血清水平显着升高（p<0.001），与观察到的核 survivin 和 fibulin-3 表达水平一致。在区分肺癌和 MPM 方面，Fibulin-3 被确定比 survivin 具有更高的价值 (p˂0.05)。Survivin 和 fibulin-3 可能是肺癌和胸膜癌的有用诊断标志物，fibulin-3 表达在区分肺癌与 MPM 方面特别有用。试验注册：ClinicalTrials.gov 标识符：NCT04413292：https://clinicaltrials.gov/ct2/show/NCT04413292，追溯注册。