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Synedrella nodiflora (L.) Gaertn Populations in Sumatra Island Showed Low Genetic Differences: A study based on the intergenic spacer atpB – rbcL
IOP Conference Series: Earth and Environmental Science Pub Date : 2020-11-21 , DOI: 10.1088/1755-1315/593/1/012035
Agus Hery Susanto 1 , Agus Nuryanto 1 , Budi Setiadi Daryono 2
Affiliation  

Previous study on Synedrella nodiflora (L.) Gaertn populations in Java Island showed both very low haplotype and nucleotide diversity, and at the same time revealed high connectivity among the populations. Sumatra Island, which is like Java Island located in Sunda Shelf, has been subjected to relatively increasing human population and overexploitation of natural resources in a few last decades. This condition put the island of being vulnerable to terrestrial ecosystem changes that potentially influence the existing populations of S. nodiflora. Hence, this study aimed to assess genetic differences among S. nodiflora populations in Sumatra Island using intergenic spacer (IGS) atpB – rbcL. This molecular marker has been used in the population genetic study of some plant species. In this study we collected randomly 20 individuals from four different locations in Sumatra. The results showed, based on IGS atpB – rbcL sequences of 860 bp length, that only two haplotypes were found. One of them was the same haplotype mostly found in Java Island, and the other showed some base substitutions. Low genetic differences indicating high connectivity among populations of S. nodiflora in Sumatra Island is observed.



中文翻译:

Synedrella nodiflora (L.) Gaertn 种群在苏门答腊岛的遗传差异较低:基于基因间间隔子 atpB – rbcL 的研究

先前对爪哇岛Synedrella nodiflora (L.) Gaertn 种群的研究显示出非常低的单倍型和核苷酸多样性,同时揭示了种群之间的高度连通性。苏门答腊岛就像位于巽他大陆架的爪哇岛一样,在过去的几十年里,人口相对增加,自然资源被过度开发。这种情况使该岛容易受到陆地生态系统变化的影响,这些变化可能会影响S. nodiflora的现有种群。因此,本研究旨在使用基因间隔区 (IGS) atp B – rbc评估苏门答腊岛S. nodiflora种群之间的遗传差异L. 该分子标记已用于一些植物物种的群体遗传研究。在这项研究中,我们从苏门答腊的四个不同地点随机收集了 20 个人。结果表明,基于 860 bp 长度的 IGS atp B – rbc L 序列,仅发现了两种单倍型。其中一个与爪哇岛最常见的单倍型相同,另一个显示出一些碱基替换。观察到低遗传差异表明苏门答腊岛S. nodiflora种群之间的高度连通性。

更新日期:2020-11-21
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