An innovative basic fibroblast growth factor (bFGF)-loaded polycaprolactone (PCL) fibrous membrane with highly aligned structure is developed for guided tissue regeneration (GTR). The aligned membrane is fabricated by electrospinning. In order to make efficient use of bFGF, PCL electrospun fibrous membrane is firstly surface-coated by self-polymerization of dopamine, and followed by immobilization of heparin via covalent conjugation to the polydopamine (PDA) layer. Subsequently, bFGF is loaded by binding to heparin. The loading yield of bFGF on heparin-immobilized PDA-coated PCL membrane significantly increases to around 7 times as compared with that of pure PCL membrane. NIH-3T3 cells show an enhanced proliferation and exhibit a stretched morphology aligned along the direction of the fibers on the aligned membranes. However, aligned bFGF-loaded PCL membrane exhibit a similar morphology but a highest cell density prolonged till 9 days. The synergetic effect of growth factor and topography would effectively regulate cell proliferation.

开发了一种具有高度对齐结构的创新型基础成纤维细胞生长因子 (bFGF) 负载聚己内酯 (PCL) 纤维膜，用于引导组织再生 (GTR)。对齐的膜是通过静电纺丝制造的。为了有效利用 bFGF，PCL 电纺纤维膜首先通过多巴胺的自聚合进行表面涂层，然后通过与聚多巴胺 (PDA) 层的共价结合固定肝素。随后，通过与肝素结合加载 bFGF。与纯 PCL 膜相比，bFGF 在肝素固定化 PDA 涂层 PCL 膜上的负载率显着增加至 7 倍左右。NIH-3T3 细胞显示出增强的增殖并表现出沿排列膜上纤维方向排列的拉伸形态。然而，对齐的装载 bFGF 的 PCL 膜表现出相似的形态，但最高细胞密度延长至 9 天。生长因子和地形的协同作用将有效地调节细胞增殖。