In this study, valuable polyhydroxynaphthoquinone (PHNQ) pigments were recovered from sea urchin food waste and were investigated as a potential bioactive ingredient for cosmeceuticals. The crude PHNQ pigment extract from 4 Vietnam sea urchins, Diadema setosum, Diadema savignyi, Stomopneustes variolaris, and Tripneustes gratilla, exhibited effective 2,2-diphenyl-1-picryl-hydrazyl-hydrate scavenging activity, tyrosinase inhibitory activity, and antibacterial activity. The moisturizing cream with 0.5% of PHNQ pigments from D. setosum and Tripneustes gratilla sea urchins showed no dermal irritation over 14 days of mouse skin test. Four major active components in PHNQ were identified via high-performance liquid chromatography coupled with diode array detector and mass spectrometry. Echinochrome A contributed considerably to the antioxidant activity of the extracts while those containing echinochrome A and spinochrome E were significantly active against various bacteria. The promising results laid the foundation for establishing a novel process from food waste to innovative biomaterial and formulating eco-friendly skincare products with PHNQ components from sea urchins as precious ingredient.

在这项研究中，从海胆食物垃圾中回收了有价值的聚羟基萘醌（PHNQ）颜料，并对其进行了研究，将其作为药妆的潜在生物活性成分。来自4个越南海胆，硬皮水蚤（Diadema setosum），硬叶水蚤（Diadema savignyi），变异气单胞菌（Stomopneustes variolaris）和中游曲霉（Tripneustes gratilla）的粗PHNQ色素提取物表现出有效的2,2-二苯基-1-picryl-肼基水合物清除活性，酪氨酸酶抑制活性和抗菌活性。与来自PHNQ颜料的0.5％的保湿霜D. setosum和Tripneustes gratilla在小鼠皮肤测试的14天中，海胆没有皮肤刺激。PHNQ中的四个主要活性成分是通过高效液相色谱与二极管阵列检测器和质谱联用确定的。Echinochrome A对提取物的抗氧化活性有很大贡献，而含有Echinochrome A和spinochrome E的提取物则对各种细菌具有显着的活性。令人鼓舞的结果为建立从食物垃圾到创新生物材料的新工艺奠定了基础，并以海胆的PHNQ成分为珍贵成分配制了环保型护肤产品。