Two novel flavonoids (1, 2) and 3 known compounds (3-5) were isolated from the flowers and whole plant of Tagetes patula L., and their structures were elucidated by means of ultra-high performance liquid chromatography with electrospray ionization, coupled to quadrupole-time-of-flight/mass spectrometry, ¹H and ¹³C-nuclear magnetic resonance (NMR), as well as 2-dimensional-NMR (heteronuclear single quantum correlation and heteronuclear multiple bond correlation) and chemical methods. In addition, all the compounds were examined for their neuroprotective action on the injury of SH-SY5Y cells induced by glutamate, indicating that the protective effect of these compounds on glutamate-induced SH-SY5Y cell was marigold biflavone > patuletin > quercetin > kaempferol-3-O-β-d-glucoside > patuletin-3-O-α-l-arabinopyranoside. Thus, it could be concluded that flavonoids played a key role in the neuroprotective action of T. patula.

两个新的黄酮类化合物（1，2）和3种已知化合物（3 - 5）从花和全植物中分离孔雀草L.，和它们的结构通过电喷雾离子化的超高效液相色谱法阐明，耦合到四极杆飞行时间/质谱，¹ H和¹³C核磁共振（NMR）以及二维NMR（异核单量子相关性和异核多键相关性）和化学方法。此外，检查了所有化合物对谷氨酸诱导的SH-SY5Y细胞损伤的神经保护作用，表明这些化合物对谷氨酸诱导的SH-SY5Y细胞的保护作用是万寿菊黄酮> patuletin>槲皮素>山ka酚- 3 -O-β - d-葡萄糖苷>patuletin-3 -O-α - l-阿拉伯吡喃糖苷。因此，可以得出结论，类黄酮在舌草的神经保护作用中起关键作用。