For experiment one, blood samples were obtained from 200 gilts at 90, 120, 150, 180, and 200 days of age. Serum samples from the 30 youngest (166.1 ± 0.7 days) and 30 oldest (198.8 ± 0.6 days) gilts exhibiting estrus by 200 days, and a further 18 gilts that remained anestrus at 200 days, were assayed for serum concentrations of anti-Mullerian hormone (AMH) and estradiol (E2). Gilts younger at puberty had higher (p < 0.05) AMH levels than those older at puberty, and both groups had higher AMH levels than anestrus gilts (p < 0.05). Regardless of age, serum E2 was higher (p < 0.05) in gilts that achieved puberty than in gilts remaining anestrus. At spontaneous pubertal estrus detection, there was no effect of pubertal age on the number of preovulatory ovarian follicles. For experiment two, 152 prepubertal gilts received an intramuscular (IM) injection of 400 IU eCG plus 200 IU hCG and then received fence-line boar contact to detect estrus onset. Serum AMH concentrations were higher (p < 0.05) in the first 25 gilts to exhibit puberty than the last 28 gilts, with the first gilts also having more preovulatory follicles (p < 0.0001). Taken together, these data support an association between serum AMH concentrations and degree of physiological maturity and ovarian follicular development in gilts.

中文翻译：

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母猪血清抗Mullerian激素和雌二醇的浓度及其青春期年龄

对于实验一，从90、120、150、180和200日龄的200头母猪获得血样。分析了30个最年轻（166.1±0.7天）和30个最老（198.8±0.6天）后备母猪在200天前出现发情的血清样本，以及另外18个在200天时仍处于发情状态的后备母猪的抗Mullerian激素的血清浓度（AMH）和雌二醇（E2）。母猪年轻在青春期有较高（p比在青春期那些年长<0.05）AMH水平，两组具有比乏母猪（较高AMH水平p <0.05）。不论年龄，血清E2均较高（p达到青春期的后备母猪比剩下的处于发情期的后备母猪<0.05。自发青春期发情时，青春期对排卵前卵巢卵泡的数量没有影响。对于实验二，152个青春期前小母猪接受了400 IU eCG加上200 IU hCG的肌肉注射（IM），然后接受围栏公猪接触以检测发情发作。前25个小母猪的血清AMH浓度高于青春期的小母猪（p <0.05），而后一个小母猪的排卵前卵泡也较多（p <0.0001）。综上所述，这些数据支持了血清AMH浓度与后备母猪生理成熟度和卵巢卵泡发育之间的关联。