Transgenic re‐expression enables unbiased investigation of T‐cell receptor (TCR)‐intrinsic characteristics detached from its original cellular context. Recent advancements in TCR repertoire sequencing and development of protocols for direct cloning of full TCRαβ constructs now facilitate large‐scale transgenic TCR re‐expression. Together, this offers unprecedented opportunities for the screening of TCRs for basic research as well as clinical use. However, the functional characterisation of re‐expressed TCRs is still a complicated and laborious matter. Here, we propose a Jurkat‐based triple parameter TCR signalling reporter endogenous TCR knockout cellular platform (TPR^KO) that offers an unbiased, easy read‐out of TCR functionality and facilitates high‐throughput screening approaches.

中文翻译：

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用于高通量和可靠研究 TCR 功能和生物学的 AT 细胞报告平台

转基因再表达能够对 T 细胞受体 (TCR) 的内在特征进行公正的研究，使其脱离其原始细胞环境。TCR 库测序和直接克隆完整 TCRαβ 构建体的协议开发的最新进展现在促进了大规模转基因 TCR 再表达。总之，这为筛选 TCR 用于基础研究和临床应用提供了前所未有的机会。然而，重新表达的 TCR 的功能表征仍然是一件复杂而费力的事情。在这里，我们提出了一个基于 Jurkat 的三参数 TCR 信号报告基因内源性 TCR 敲除细胞平台 (TPR ^KO )，它提供了一个无偏、容易读取 TCR 功能并促进高通量筛选方法的方法。