The application of a magnetic field to enhance the transfection efficiency has been reported to be mainly dependent on the magnetic force generated by a magnetic field gradient to attract paramagnetic bead‐conjugated carrier and polynucleotide complexes. This strategy has the advantage of targeting a point or an area on the culture vessel. However, it is difficult to target deeply placed tissues in vivo. Uniform magnetic field‐correlated effect is applicable to such a purpose. Here, we attempted to establish a novel procedure for uniform magnetic field‐dependent enhancement of transfection efficiency. We examined the effect of a 1.5 mT uniform magnetic field on cellular reactive oxygen species (ROS) level and transfection efficiency mediated by a ROS‐sensitive transfection carrier. Our experimental results revealed that a 1.5 mT uniform magnetic field transiently decreased cellular ROS levels and strongly enhanced transfection efficiency mediated by polyethylenimine (PEI). The uniform magnetic field‐dependent enhancement of PEI‐mediated in vivo transfection was confirmed in the livers of mice. Local intensification of a uniform magnetic field in a culture dish resulted in selective gene delivery into cells on the target area. Although further examination and improvement are necessary for this procedure, our findings provide a novel option for spatial control of gene delivery.

中文翻译：

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利用均匀磁场选择性传递基因的新策略

据报道，施加磁场以提高转染效率主要取决于磁场梯度所产生的磁力，以吸引顺磁珠缀合的载体和多核苷酸复合物。该策略的优势是瞄准培养容器上的一个点或一个区域。但是，很难在体内靶向深层组织。均匀的磁场相关效应适用于此目的。在这里，我们试图建立一种新颖的程序，用于均匀依赖磁场增强转染效率。我们检查了1.5 mT均匀磁场对由ROS敏感的转染载体介导的细胞活性氧（ROS）水平和转染效率的影响。我们的实验结果表明，结果为1。5 mT的均匀磁场可暂时降低细胞ROS的水平，并大大提高聚乙烯亚胺（PEI）介导的转染效率。在小鼠肝脏中证实了PEI介导的体内转染的均匀磁场依赖性增强。培养皿中均匀磁场的局部增强导致选择性基因传递到靶区域的细胞中。尽管对该程序需要进一步的检查和改进，但我们的发现为基因传递的空间控制提供了新的选择。培养皿中均匀磁场的局部增强导致选择性基因传递到靶区域的细胞中。尽管对该程序需要进一步的检查和改进，但我们的发现为基因传递的空间控制提供了新的选择。培养皿中均匀磁场的局部增强导致选择性基因传递到靶区域的细胞中。尽管对该程序需要进一步的检查和改进，但我们的发现为基因传递的空间控制提供了新的选择。