Marsdenia tenacissima (Roxb.) Wight et Arn. (M. tenacissima) is considered an anticancer medicine in traditional Chinese medicine, which is extensively used in clinical application since it has great therapeutic effects. Currently, although a number of articles have examined M. tenacissima in terms of its pharmacology and quality control, few have investigated the in vivo mechanism of M. tenacissima active ingredients. Previously, we have studied the pharmacokinetics of eight active ingredients after oral administration of M. tenacissima extracts in rat plasma. This study constructed a new scientific ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) approach to simultaneously quantify the contents of tenacissosides B, G, H and I, cryptochlorogenic acid, chlorogenic acid, neochlorogenic acid and caffeic acid in rats orally administered M. tenacissima extract. The proposed approach was successfully used for investigating the distributions of those eight analytes in rat tissues, with digoxin being used as an internal control. The Eclipse Plus C₁₈ RRHD column was used for determination at a column temperature of 30°C. The mobile phase system consisted of acetonitrile and water (supplemented with 0.1% formic acid) under optimal gradient elution conditions. Afterwards, this approach was validated according to the requirements for the analysis of biological samples developed by the US Food and Drug Administration, including precision, accuracy, stability and matrix effects. Based on tissue distribution analysis, those eight analytes showed rapid distribution within all the tested tissues. With regard to organic acid distribution, it followed the order stomach > liver > kidney > small intestine > lung > spleen > heart, whereas the four steroids followed the order stomach > lung > spleen > small intestine > liver > kidney > heart. The present study lays the theoretical foundation for the use and development of M. tenacissima in clinical practice.

中文翻译：

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通过UPLC-MS / MS口服检测到的马氏疟原虫提取物在大鼠组织中的八种生物活性成分的分布

Marsdenia tenacissima（Roxb。）Wight et Arn。（M. tenacissima）（M. tenacissima）被认为是中药中的一种抗癌药物，由于它具有很好的治疗作用，因此在临床上被广泛使用。当前，尽管许多文章已经从其药理学和质量控制方面检查了tenacissima，但是很少有人研究tenacissima活性成分的体内机制。以前，我们已经研究了口服M. tenacissima后八种有效成分的药代动力学。在大鼠血浆中提取。这项研究构建了一种新的科学超高效液相色谱-串联质谱（UPLC-MS / MS）方法，用于同时定量测定水中的Tenacissosides B，G，H和I，隐绿原酸，绿原酸，新绿原酸和咖啡酸的含量。大鼠口服给予tenacissima提取物。所提出的方法已成功用于调查这八种分析物在大鼠组织中的分布，地高辛被用作内部对照。Eclipse Plus C ₁₈RRHD色谱柱用于在30°C的色谱柱温度下进行测定。在最佳梯度洗脱条件下，流动相系统由乙腈和水（补充有0.1％甲酸）组成。之后，根据美国食品和药物管理局开发的生物样品分析要求，包括精确度，准确性，稳定性和基质效应，对这种方法进行了验证。根据组织分布分析，这八种分析物显示出在所有测试组织中的快速分布。关于有机酸的分布，它遵循胃>肝>肾>小肠>肺>脾>心脏的顺序，而四种类固醇遵循胃>肺>脾>小肠>肝>肾>心脏的顺序。Tenacissima M.在临床实践中。