Proteins play important roles in mediating various forms of life activities. The development of convenient and reliable strategies for simultaneous recognition of multiple proteins is meaningful for early diagnostics of diseases. Herein, we develop a differential colorimetric sensor array, which is made of nonspecific single-stranded DNA (ssDNA) as receptors and gold nanoparticles (AuNPs) as colorimetric probes for multiple identification of the nine proteins. Upon the addition of analyte proteins, different interactions between various proteins and ssDNA lead to the desorption of different amounts of ssDNA from the AuNP surface, thus resulting in AuNP aggregation to varying degrees in salt environment, which in turn leads to the colorimetric signal change of AuNPs. On the basis of the principle, nine proteins (i.e., bovine serum albumin (BSA), horseradish peroxidase (HRP), pepsin (Pep), hemoglobin (Hem), egg white albumin (EA), trypsin (Try), myoglobin (Myo), cytochrome C (Cyt-C), and concanavalin (Con)) at low concentration (20 nM) were well discriminated by the sensor array.

蛋白质在介导各种形式的生命活动中起着重要作用。同时识别多种蛋白质的便捷可靠策略的开发对疾病的早期诊断具有重要意义。本文中，我们开发了一种差分比色传感器阵列，该阵列由非特异性单链DNA（ssDNA）作为受体和金纳米颗粒（AuNPs）作为比色探针，可对9种蛋白质进行多次鉴定。添加分析物蛋白质后，各种蛋白质与ssDNA之间的不同相互作用导致从AuNP表面解吸出不同量的ssDNA，从而导致AuNP在盐环境中聚集到不同程度，进而导致比色信号的变化。 AuNPs。根据该原理，九种蛋白质（即牛血清白蛋白（BSA），