The assessment of behavioral outcomes is a central component of neuroscientific research, which has required continuous technological innovations to produce more detailed and reliable findings. In this article, we provide an in-depth review on the progress and future implications for three model organisms (mouse, rat, and Drosophila) essential to our current understanding of behavior. By compiling a comprehensive catalog of popular assays, we are able to compare the diversity of tasks and usage of these animal models in behavioral research. This compilation also allows for the evaluation of existing state-of-the-art methods and experimental applications, including optogenetics, machine learning, and high-throughput behavioral assays. We go on to discuss novel apparatuses and inter-species analyses for centrophobism, feeding behavior, aggression and mating paradigms, with the goal of providing a unique view on comparative behavioral research. The challenges and recent advances are evaluated in terms of their translational value, ethical procedures, and trustworthiness for behavioral research.

行为结果的评估是神经科学研究的重要组成部分，需要不断的技术创新才能产生更详细和可靠的发现。在本文中，我们对三种模型生物（小鼠，大鼠和果蝇）的进展和未来影响进行了深入综述。）对于我们当前对行为的理解至关重要。通过汇编流行测定的综合目录，我们能够比较行为研究中这些动物模型的任务多样性和用途。该汇编还可以评估现有的最新方法和实验应用，包括光遗传学，机器学习和高通量行为分析。我们将继续讨论针对恐同症，进食行为，侵略性和交配范式的新颖仪器和种间分析，目的是为比较行为研究提供独特的见解。评估挑战和最新进展的方式包括其转化价值，道德程序和行为研究的可信度。