The production of tambaqui Colossoma macropomum has recently reached a milestone, being considered the main native species produced in South American continental waters. Despite the importance of this fish, its immunity is not well understood. In this study we established some patterns of innate immunity for the species via two experiments. Both studies evaluated the fish in the absence (intraperitoneal saline) or presence (intraperitoneal, 3 x 10⁷ CFU/mL of Aeromonas hydrophila at 0.1 mL/10 g of living weight) of infection at 5 points over time-course of 14 days (0 h, 6 h, 24 h, 7 d, 14 d). In the first experiment, the partial gene sequences and gene expression of IL-1β, IRAK-1, C3, C4, lysozyme, IL-10, HSP70 and β-actin were determined in the main secondary lymphoid organs of fish: the spleen and head kidney. The second study was performed to analyse the alternative complement pathway ACH₅₀ in serum to support the elucidation of C3 gene expression. Results of the gene expression assays showed a tendency towards up-regulation of immune genes in infected fish in early phases of infection (mostly around 6 h and 24 h) and in the chronic phase (7 d and 14 d), with the exception of HSP70 which showed a down-regulation in infected fish. Our results also suggested that lysozyme was evolved in both pro- and anti-inflammatory activities. For genes of the complement system, it was demonstrated that C4 regulation followed the tendency of pro-inflammatory genes. However, the C3 gene was, surprisingly, not expressed in most fish and this corroborated with the results of the complement system activity in serum that also did not show activity in most fish. The possible reasons for the regulation of gene expression and association with fish disease are addressed in this paper.

tambaqui巨大巨像的生产最近达到了一个里程碑，被认为是南美大陆水域生产的主要本地物种。尽管这条鱼很重要，但对它的免疫力却知之甚少。在这项研究中，我们通过两个实验建立了该物种固有免疫的一些模式。两项研究均评估了鱼类是否存在（腹膜内盐水）或是否存在（腹膜内，3 x 10 ⁷  CFU / mL嗜水气单胞菌）在14天（0 h，6 h，24 h，7 d，14 d）的时间过程中，在5点感染了0.1 mL / 10 g活重的感染。在第一个实验中，确定了鱼的主要次要淋巴器官中的IL-1β，IRAK-1，C3，C4，溶菌酶，IL-10，HSP70和β-肌动蛋白的部分基因序列和基因表达。头肾。进行第二项研究以分析替代补体途径ACH ₅₀在血清中支持C3基因表达的阐明。基因表达分析的结果表明，在感染的早期（主要在6 h和24 h左右）和慢性阶段（7 d和14 d），受感染鱼的免疫基因有上调的趋势。 HSP70在受感染的鱼类中表达下调。我们的结果还表明，溶菌酶的促炎和抗炎活性均得到了发展。对于补体系统的基因，已证明C4调节遵循促炎基因的趋势。然而，令人惊讶的是，C3基因在大多数鱼类中均未表达，这与血清中补体系统活性的结果相符，而该结果在大多数鱼类中也未显示出活性。