The detection of extracellular vesicles, or exosomes are important mediators in intercellular communication and often play a role in cancer progression. CD63 is a key exosomal protein due to its distinctive cellular functions and association with many cancers. This describes a label-free electrochemiluminescence (ECL) nanoimmunosensor for the detection of CD63 protein over mesoporous carbon screen-printed electrode (MC-SPE) modified with novel nanocomposite of carbon nanochips (CNCs), iron oxide (Fe₃O₄) and nafion (NAF) . Fourier-transform infrared spectroscopy and field emission scanning electron microscopy were used to analyse nanocomposite. All the analytical performance of fabricated CD63 immunosensor were conducted applying ECL. In spite of the simple fabrication strategies utilized, the fabricated immunosensor showcased a broad linear range to detect CD63 from 100 fg mL^-1 to 10 ng mL^-1, with a limit of detection of 100 fg mL^-1, excellent selectivity, interference-resistance capability and potential to detect CD63 in real clinical samples.

细胞外囊泡或外泌体的检测是细胞间通讯的重要介体，通常在癌症进展中起作用。由于CD63具有独特的细胞功能并与许多癌症相关，因此它是一种关键的外泌体蛋白。本文描述了一种无标记的电化学发光（ECL）纳米免疫传感器，用于在用新型纳米碳复合材料（CNC），氧化铁（Fe ₃ O ₄）修饰的介孔碳丝网印刷电极（MC-SPE）上检测CD63蛋白。）和nafion（NAF）。傅里叶变换红外光谱和场发射扫描电子显微镜用于分析纳米复合材料。制备的CD63免疫传感器的所有分析性能均采用ECL进行。尽管采用了简单的制备策略，但制成的免疫传感器仍显示出宽广的线性范围，可检测从100 fg mL ^-1到10 ng mL ^-1的CD63 ，检测限为100 fg mL ^-1，具有出色的选择性，干扰-耐药能力和检测实际临床样品中CD63的潜力。