Bacterial adhesion infection caused by medical materials in clinical application has become a serious threat, and it urgently needs new strategies to deal with these clinical challenges. The purpose of this study is to explore the effectiveness of surface-decorated aryl (β-amino) ethyl ketones (AAEK), a promising sorting enzyme A (SrtA) inhibitor of Staphylococcus aureus, to improve the anti-adhesion ability of biomaterials. AAEK was covalently grafted onto cellulose films (CF) via copper-catalyzed azide-alkyne 1, 3-dipolar cycloaddition click reaction. The data of contact angle measurements, ATR-FTIR and XPS proved the successful covalent attachment of AAEK-CF, and the antimicrobial efficacy of AAEK coating was assessed by CFUs, crystal violet staining, scanning electron microscopy and Living/Dead bacteria staining assay. The results illustrated that AAEK-CF exhibited excellent anti-adhesion ability to Staphylococcus aureus, and significantly reduced the number of bacteria adhering to the film. More importantly, AAEK-CF could hinder the formation of bacterial biofilm. Furthermore, AAEK-CF indicated no cytotoxicity to mammalian cells, and the cells could grow normally on the modified surface. Hence, our present work demonstrated that the grafting of the SrtA inhibitor-AAEK onto cellulose films enabled to combat bacterial biofilm formation in biomedical applications.

在临床应用中，由医用材料引起的细菌粘附感染已成为严重的威胁，因此迫切需要新的策略来应对这些临床挑战。这项研究的目的是探讨表面修饰的芳基（β-氨基）乙基酮（AAEK）的有效性，这是一种有前景的金黄色葡萄球菌分选酶A（SrtA）抑制剂，以提高生物材料的抗粘连能力。通过铜催化的叠氮化物-炔烃1，3-偶极环加成点击反应，将AAEK共价接枝到纤维素膜（CF）上。接触角测量数据，ATR-FTIR和XPS证明了AAEK-CF的成功共价连接，并通过CFU，结晶紫染色，扫描电子显微镜和活/死细菌染色测定法评估了AAEK涂层的抗菌功效。结果表明，AAEK-CF对金黄色葡萄球菌具有优异的抗黏附能力。，并大大减少了粘附在薄膜上的细菌数量。更重要的是，AAEK-CF可能会阻碍细菌生物膜的形成。此外，AAEK-CF对哺乳动物细胞没有细胞毒性，并且细胞可以在修饰的表面上正常生长。因此，我们目前的工作表明，将SrtA抑制剂-AAEK接枝到纤维素膜上可以对抗生物医学应用中细菌生物膜的形成。