The aim of the present study was to evaluate terpenes degradation rate in the rumen fluid from adapted and non-adapted animals. Four castrated healthy animals, two rams and two bucks, were used. Animals were daily orally dosed for 2 weeks with 1 g of each of the following terpenes, α-pinene, limonene and β-caryophyllene. At the end of each week, rumen fluid (RF) samples were assayed in vitro for their potential to degrade terpenes over time. For each animal, a 10 mL reaction medium (RM) at a ratio 1:9 (v/v) was prepared and a terpenes solution at a concentration of 100 μg/ml each, was added in each RM tube. Tubes were incubated at 39 °C under anaerobic conditions and their contents sampled at 0, 2, 4, 8, 21 and 24 h. RF could degrade terpenes as it was shown by the significantly (P < 0.05) higher overall degradation rates. Individual terpene degradation rates, were significantly (P < 0.05) higher in week 5 for limonene and marginally (P = 0.083) higher also in week 5 for α-pinene. In conclusion, the findings of the present preliminary study suggest that terpenes can be degraded in the rumen fluid.

本研究的目的是评估适应和不适应动物的瘤胃液中萜烯的降解率。使用了四只cast割的健康动物，两只公羊和两美元。每天给动物口服2周，每次1克以下萜烯，α-pine烯，li烯和β-石竹烯。在每周结束时，体外分析瘤胃液（RF）样品随时间降解萜烯的潜力。对于每只动物，制备比例为1：9（v / v）的10 mL反应介质（RM），并在每个RM管中加入浓度分别为100μg/ ml的萜烯溶液。将试管在厌氧条件下于39°C孵育，并在0、2、4、8、21和24 h取样。RF可以降解萜烯，正如总体降解率显着提高（P <0.05）所表明的那样。柠檬烯的第5周个体萜降解速率显着提高（P <0.05），而α--烯的第5周显着提高（P = 0.083）。总之，目前的初步研究结果表明萜烯可以在瘤胃液中降解。