The objective of this study was to determine the efficacy of the Fassisi®BoviPreg visual test kit (Fassisi®BoviPreg) in determining early pregnancies in cows by measuring the pregnancy-associated glycoprotein (PAG) in whole blood and blood serum. The study was conducted on 50 cows, the artificial insemination (AI) dates of which were designated as day 0. Pregnancy diagnosis was performed with transrectal ultrasonography (USG), and serum samples were simultaneously collected and used with Fassisi®BoviPreg to determine pregnancies on 30 days after AI. The results of the Fassisi®BoviPreg test on serum and whole blood samples, respectively, on 30 days after AI were as follows: sensitivity, 61.54% and 50.0%; specificity, 79.17% and 75%; accuracy, 70.0% and 62.0%; positive predictive values, 76.2% and 68.4%; negative predictive values, 65.5% and 58.1%; false-positive diagnoses, 23.8% and 31.6%; and false-negative diagnoses, 34.5% and 41.9%. On day 50 after AI, sensitivities were 63.64% and 50.0%, specificities were 100.0% and 100.0%, and accuracies were 75.0% and 65.62% in serum and whole blood, respectively. Higher pregnancy rates were obtained using Fassisi®BoviPreg in cows with ≥ 5 ng/mL P4 (P < 0.001). In conclusion, the results from the Fassisi®BoviPreg tests on cows on 30 and 50 days after AI showed that the use of blood serum is more accurate and suitable than that of whole blood. The results also showed a higher confidence level in specificity 50 days after AI.

这项研究的目的是通过测量全血和血清中与妊娠相关的糖蛋白（PAG）来确定Fassisi®BoviPreg视觉检测试剂盒（Fassisi®BoviPreg）在确定牛早孕中的功效。该研究针对50头母牛进行了人工授精（AI）日期指定为第0天。使用经直肠超声（USG）进行妊娠诊断，同时收集血清样本，并与Fassisi®BoviPreg一起用于确定妊娠。 AI后30天。AI后30天，分别对血清和全血样本进行Fassisi®BoviPreg检测的结果如下：敏感性分别为61.54％和50.0％；特异性分别为79.17％和75％；准确度分别为70.0％和62.0％；阳性预测值分别为76.2％和68.4％；阴性预测值分别为65.5％和58.1％；假阳性诊断率分别为23.8％和31.6％；假阴性诊断率分别为34.5％和41.9％。AI后第50天，血清和全血的敏感性分别为63.64％和50.0％，特异性为100.0％和100.0％，准确度分别为75.0％和65.62％。使用Fassisi®BoviPreg在P5≥5 ng / mL的母牛中获得更高的妊娠率（P  <0.001）。总之，在AI后30天和50天对奶牛进行Fassisi®BoviPreg测试的结果表明，使用血清比全血更准确，更合适。结果还显示，AI后50天对特异性的置信度更高。