Long non-coding RNAs (lncRNAs) are a group of non-protein-coding RNAs which are longer than 200 nucleotides. LncRNAs play important roles in epigenetic modification, transcription and post-transcriptional regulation, maintenance of normal tissue development and differentiation. LncRNA could serve as a biomarker for diagnosis and prognosis as well as a molecular target for therapy in oral squamous cell carcinoma (OSCC). Therefore, we have determined the expression profile of 5-lncRNAs namely UCA1, TUG1, HOTAIR, MALAT1, and H19 by quantitative real-time PCR in tumor tissues and adjacent normal tissue of 32 OSCC patients. To determine the expression, methylation status and genomic alterations in lncRNAs across pan-cancer, TCGA datasets were analyzed by UALCAN, MEXPRESS and cBioPortal database. Then, we determined the association between lncRNA expression and clinicopathological attributes of patients by Spearman’s rank test. Expression of UCA1 and TUG1 genes was up-regulated in 54.83% and 53.12% OSCC tumors, respectively. Importantly, expression of MALAT1 and H19 was down-regulated in tumor tissues of 62.5% and 81.25% respectively of OSCC patients. Except for MALAT1, our experimental data showed concordance with the TCGA analysis. Expression of HOTAIR in OSCC tumors was positively correlated with tumor volume, whereas MALAT1 and H19 negatively correlated with the smoking status of patients.

中文翻译：

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H19长链非编码RNA在口腔鳞状细胞癌中的表达下调

长链非编码 RNA (lncRNA) 是一组长度超过 200 个核苷酸的非蛋白质编码 RNA。LncRNA 在表观遗传修饰、转录和转录后调控、维持正常组织发育和分化中发挥重要作用。LncRNA 可作为诊断和预后的生物标志物以及口腔鳞状细胞癌 (OSCC) 治疗的分子靶点。因此，我们通过实时定量 PCR 在 32 名 OSCC 患者的肿瘤组织和邻近正常组织中确定了 5-lncRNA，即 UCA1、TUG1、HOTAIR、MALAT1 和 H19 的表达谱。为了确定泛癌中 lncRNA 的表达、甲基化状态和基因组改变，通过 UALCAN、MEXPRESS 和 cBioPortal 数据库分析了 TCGA 数据集。然后，我们通过 Spearman 等级检验确定了 lncRNA 表达与患者临床病理属性之间的关联。UCA1 和 TUG1 基因的表达分别在 54.83% 和 53.12% 的 OSCC 肿瘤中上调。重要的是，MALAT1 和 H19 的表达在 OSCC 患者的肿瘤组织中分别下调 62.5% 和 81.25%。除了 MALAT1，我们的实验数据与 TCGA 分析一致。HOTAIR在OSCC肿瘤中的表达与肿瘤体积呈正相关，而MALAT1和H19与患者的吸烟状况呈负相关。MALAT1和H19的表达在OSCC患者的肿瘤组织中分别下调62.5%和81.25%。除了 MALAT1，我们的实验数据与 TCGA 分析一致。HOTAIR在OSCC肿瘤中的表达与肿瘤体积呈正相关，而MALAT1和H19与患者的吸烟状况呈负相关。MALAT1和H19的表达在OSCC患者的肿瘤组织中分别下调62.5%和81.25%。除了 MALAT1，我们的实验数据与 TCGA 分析一致。HOTAIR在OSCC肿瘤中的表达与肿瘤体积呈正相关，而MALAT1和H19与患者的吸烟状况呈负相关。