Peripheral nerve networks (PNNs) play a vital role in the neural recovery after spinal cord injury (SCI). Electroacupuncture (EA), as an alternative medicine, has been widely used in SCI and was proven to be effective on neural functional recovery. In this study, the interaction between PNNs and semaphrin3A (Sema3A) in the recovery of the motor function after SCI was observed, and the effect of EA on them was evaluated. After the establishment of the SCI animal model, we found that motor neurons in the ventral horn of the injured spinal cord segment decreased, Nissl bodies were blurry, and PNNs and Sema3A as well as its receptor neuropilin1 (NRP1) aggregated around the central tube of the gray matter of the spinal cord. When we knocked down the expression of Sema3A at the damage site, NRP1 also downregulated, importantly, PNNs concentration decreased, and tenascin-R (TN-R) and aggrecan were also reduced, while the Basso-Beattie-Bresnahan (BBB) motor function score dramatically increased. In addition, when conducting EA stimulation on Jiaji (EX-B2) acupoints, the highly upregulated Sema3A and NRP1 were reversed post-SCI, which can lessen the accumulation of PNNs around the central tube of the spinal cord gray matter, and simultaneously promote the recovery of motor function in rats. These results suggest that EA may further affect the plasticity of PNNs by regulating the Sema3A signal and promoting the recovery of the motor function post-SCI.

中文翻译：

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电针通过抑制信号素3A信号转导和上调外周神经网络改善脊髓损伤大鼠的运动功能

周围神经网络 (PNN) 在脊髓损伤 (SCI) 后的神经恢复中起着至关重要的作用。电针（EA）作为一种替代药物，已被广泛用于脊髓损伤，并被证明对神经功能恢复有效。本研究观察了 PNNs 与 semaphrin3A（Sema3A）在 SCI 后运动功能恢复中的相互作用，并评估了 EA 对其的影响。SCI动物模型建立后，我们发现损伤脊髓节段腹角运动神经元减少，尼氏体模糊，PNNs和Sema3A及其受体neuropilin1（NRP1）聚集在脊髓中央管周围。脊髓灰质。当我们在损伤部位敲低 Sema3A 的表达时，NRP1 也下调，重要的是，PNNs 浓度降低，生腱蛋白-R (TN-R) 和蛋白聚糖也减少，而 Basso-Beattie-Bresnahan (BBB) 运动功能评分显着增加。此外，在对夹脊（EX-B2）穴位进行电针刺激时，高度上调的 Sema3A 和 NRP1 在 SCI 后被逆转，这可以减少脊髓灰质中央管周围 PNN 的积累，同时促进大鼠运动功能的恢复。这些结果表明 EA 可能通过调节 Sema3A 信号和促进 SCI 后运动功能的恢复来进一步影响 PNN 的可塑性。SCI后高度上调的Sema3A和NRP1被逆转，可以减少脊髓灰质中央管周围PNNs的积累，同时促进大鼠运动功能的恢复。这些结果表明 EA 可能通过调节 Sema3A 信号和促进 SCI 后运动功能的恢复来进一步影响 PNN 的可塑性。SCI后高度上调的Sema3A和NRP1被逆转，可以减少脊髓灰质中央管周围PNNs的积累，同时促进大鼠运动功能的恢复。这些结果表明 EA 可能通过调节 Sema3A 信号和促进 SCI 后运动功能的恢复来进一步影响 PNN 的可塑性。