In previous studies, we reported evidence showing that chronic cola consumption in rats impairs pancreatic metabolism of insulin and glucagon and produces some alterations typically observed in the metabolic syndrome (i.e, hyperglycemia, and hypertriglyceridemia) with an increase in oxidative stress. Of note, no apoptosis nor proliferation of islet cells could be demonstrated. In the present study, 36 male Wistar rats were divided in three groups to freely drink regular cola, light cola, or water (controls). We assessed the impact of the three different beverages in glucose tolerance, lipid levels, creatinine levels and immunohistochemical changes addressed for the expression of insulin, glucagon, PDX-1 and NGN3 in islet cells, to evaluate the possible participation of PDX-1 in the changes observed in α and β cells after 6 months of treatment. On the other hand, we assessed by stereological methods, the mean volume of islets (Vi) and three important variables, the fractional β -cell area, the cross-sectional area of alpha (A α-cell) and beta cells (A β-cell), and the number of β and α cell per body weight.

中文翻译：

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TDX-1能否解释大鼠长期饮用可乐引起的内分泌胰腺变化？全球观点

在以前的研究中，我们报道了证据，表明大鼠长期食用可乐会损害胰岛素和胰高血糖素的胰腺代谢，并会产生氧化综合征增加时通常在代谢综合征（即高血糖和高甘油三酸酯血症）中观察到的某些改变。值得注意的是，没有显示出凋亡或胰岛细胞的增殖。在本研究中，将36只Wistar雄性大鼠分为三组，以自由饮用普通可乐，淡可乐或水（对照组）。我们评估了三种不同饮料对胰岛细胞中胰岛素，胰高血糖素，PDX-1和NGN3表达的葡萄糖耐量，脂质水平，肌酐水平和免疫组化变化的影响，以评估PDX-1可能参与了胰岛细胞的表达。治疗6个月后观察到的α和β细胞发生了变化。