当前位置: X-MOL 学术J. Biomater. Sci. Polym. Ed. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Synthesis and characterization of PLGA-PEG-PLGA based thermosensitive polyurethane micelles for potential drug delivery
Journal of Biomaterials Science, Polymer Edition ( IF 3.6 ) Pub Date : 2020-12-16 , DOI: 10.1080/09205063.2020.1854413
Min Wang 1 , Jianghao Zhan 1 , Laijun Xu 2 , Yanjun Wang 1 , Dan Lu 3 , Zhen Li 1 , Jiehua Li 1 , Feng Luo 1 , Hong Tan 1
Affiliation  

Abstract

Polyurethane nanomicelle is a promising functional drug delivery system. In this work, the polyurethane (P3-PU) was synthesized from PLGA1200-PEG1450-PLGA1200 (P3, a thermosensitive and biodegradable triblock copolymer) and L-lysine ester diisocyanate (LDI). Then, reactive benzaldehyde was further imported to terminate P3-PU to obtain benzaldehyde modified polyurethane (P3-PUDA). The micelles, temperature-sensitive P3-PU nanomicelle and P3-PUDA nanomicelle, were systematically investigated, including the size, stability, temperature sensitivity, drug loading and release behavior, cytotoxic on human hepatocytes (L02), and inhibitory effect on human hepatocellular carcinoma cells (HepG2). The results show the thermosensitive behavior of the micelles can be adjusted by the terminal group. The polyurethane micelles with a uniform size between 20 nm and 30 nm showed excellent stability and good biocompatibility to L02 cells. Besides, in vitro experiments showed that Dox-loaded P3-PUDA micelles exhibited faster and higher release rate at 37 °C and better inhibitory effect on HepG2 than the Dox-loaded P3-PU micelles. Moreover, the achieved benzaldehyde modified polyurethanes also provides various possibilities to adjust further to enlarge its applications. Therefore, the polyurethane micelles will have great potential in the field of drug carriers.



中文翻译:

用于潜在药物递送的基于 PLGA-PEG-PLGA 的热敏聚氨酯胶束的合成和表征

摘要

聚氨酯纳米胶束是一种很有前途的功能性药物递送系统。在这项工作中,聚氨酯(P3-PU)从PLGA合成1200 -PEG 1450 -PLGA 1200(P3,一种热敏和可生物降解的三嵌段共聚物)和 L-赖氨酸酯二异氰酸酯 (LDI)。然后进一步导入反应性苯甲醛终止P3-PU,得到苯甲醛改性聚氨酯(P3-PUDA)。系统研究了胶束、温度敏感的 P3-PU 纳米胶束和 P3-PUDA 纳米胶束,包括大小、稳定性、温度敏感性、载药和释放行为、对人肝细胞 (L02) 的细胞毒性和对人肝细胞癌的抑制作用细胞(HepG2)。结果表明胶束的热敏行为可以通过端基进行调节。大小均一的聚氨酯胶束在 20 nm 到 30 nm 之间显示出优异的稳定性和对 L02 细胞的良好生物相容性。此外,体外实验表明,与负载 Dox 的 P3-PU 胶束相比,负载 Dox 的 P3-PUDA 胶束在 37°C 下表现出更快和更高的释放速率,对 HepG2 的抑制作用更好。此外,所获得的苯甲醛改性聚氨酯还提供了进一步调整以扩大其应用的各种可能性。因此,聚氨酯胶束在药物载体领域将具有巨大的潜力。

更新日期:2020-12-16
down
wechat
bug