CONTEXT Angelica sinensis (Oliv.) Diels (Apiaceae) (syn. Angelica polymorpha Maxim var. sinensis Oliver) processed with yellow rice wine (WAS) has a blood-supplementing effect. OBJECTIVE To establish an optimal technology for preparing water decoction of WAS (WASD), and screen blood-supplementing fractions. MATERIALS AND METHODS Ferulic acid and crude polysaccharide were used in optimizing the preparation technology for WASD through response surface methodology. The independent variables were liquid-solid ratio, soaking time, and extraction time. Eighty Kunming mice were randomly divided into normal control, model, and six intervention groups (n = 10). The intervention groups were given different WASD fractions by gavage (5 or 10 g/kg). The model intervention groups received acetylphenyl hydrazine (subcutaneous injection) and cyclophosphamide (intraperitoneal injection). Duration of study, 9 days. The components of blood-supplementing fractions were analyzed. RESULTS The optimum extraction parameters were liquid-solid ratio, 7.69:1 mL/g; soaking time, 119.78 min; and extraction time, 143.35 min. The optimal OD value was 0.8437. RBC, WBC, and Hb in the water fraction (5, 10 g/kg) and n-butanol fraction (10 g/kg) intervention groups increased significantly compared with the model group (p < 0.05). Polysaccharide and caffeic acid contents of water fraction were 252.565 and 0.346 μg/mg, respectively; ferulic acid was not detected. Caffeic acid and ferulic acid contents of n-butanol fraction were 1.187 and 0.806 μg/mg, respectively, polysaccharide was not detected. CONCLUSIONS The optimum preparation technology of WASD was obtained, and the water, n-butanol fractions were blood-supplementing fractions. This study provides a theoretical foundation for further application of WAS in the pharmaceutical industry.

中文翻译：

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基于响应面法的黄酒煎当归水煎剂补血活性成分筛选研究

上下文 当归 (Oliv.) Diels (Apiaceae) (syn. Angelica polymorpha Maxim var. sinensis Oliver) 用黄酒 (WAS) 加工，具有补血作用。目的建立WAS水煎剂（WASD）的最佳制备工艺，筛选补血组分。材料与方法 阿魏酸和粗多糖通过响应面法优化WASD的制备工艺。自变量是液固比、浸泡时间和提取时间。80只昆明小鼠随机分为正常对照组、模型组和6个干预组（n=10）。通过管饲法（5 或 10 g/kg）给予干预组不同的 WASD 分数。模型干预组接受乙酰苯肼（皮下注射）和环磷酰胺（腹腔注射）。学习期限，9 天。分析了补血组分的成分。结果最佳提取参数为液固比7.69:1 mL/g；浸泡时间，119.78 分钟；和提取时间，143.35 分钟。最佳 OD 值为 0.8437。与模型组相比，水部分（5、10 g/kg）和正丁醇部分（10 g/kg）干预组的RBC、WBC和Hb显着增加（p < 0.05）。水部分多糖和咖啡酸含量分别为252.565和0.346 μg/mg；未检测到阿魏酸。正丁醇馏分的咖啡酸和阿魏酸含量分别为 1.187 和 0.806 μg/mg，未检测到多糖。结论获得了WASD的最佳制备工艺，水、正丁醇馏分为补血馏分。本研究为WAS在制药行业的进一步应用提供了理论基础。