ABSTRACT

The wild species of Solanum are important genetic resources for the medicinal and vegetable crop species of the genus and reservoir of genes for developing new economic uses of Solanum species. In this article, the genetic diversity and systematics of 14 Solanum accessions representing 11 species growing in the southwestern highlands of Saudi Arabia were evaluated using morphological variations and molecular polymorphism generated by 13 ISSR primers. Substantial variations were scored in 26 morphological characters and reflected in the cluster analysis of the examined accessions based on morphological variation. In the cluster trees illustrating the relatedness of the examined accessions based on morphological variation and ISSR polymorphism, the accessions of S. villosum and S. nigrum were differentiated as one cluster and S. dulcamara was associated with S. sisymbriifolium. In the meantime, S. coagulans was clustered with S. macracanthum. The analysis of ISSR data confirms that S. villosum is related to S. nigrum and supports the grouping S. incanum and S. glabratum based on the morphological traits. Principle Component Analysis (PCA) analysis of morphological and ISSR data as a combined set, differentiated the examined species into four groups in a scatter plot. The differentiation of the species/populations in the PCA scatter diagram agrees with their separation in the cluster trees. The four groups are 1. S. macracanthum, S. coagulans and S. schimperianum. 2. S. glabratum and S. incanum. 3. S. dulcamara, S. sisymbriifolium and S. torvum, 4. S. nigrum, S. villosum, and S. villosum ssp. puniceum.

摘要

茄属植物的野生物种是药用和蔬菜作物物种的重要遗传资源，并且是开发茄属植物物种的新经济用途的基因库。本文研究了14种茄科植物的遗传多样性和系统学。使用13种ISSR引物产生的形态变异和分子多态性，对代表沙特阿拉伯西南高地生长的11个物种的种质进行了评估。对26个形态特征进行了实质性变异评分，并反映在基于形态变异的受检种质的聚类分析中。在群集树示出了基于形态变异和多态性ISSR所检查的种质的亲缘关系，的种质S.砂仁和龙葵分化为一个簇和S.杜尔卡马拉用相关联S. sisymbriifolium。同时，凝结链球菌与Macracanthum聚集在一起。。ISSR数据确认的分析认为S.砂仁涉及龙葵和支持分组S. incanum和S. glabratum基于该形态特征。对形态学和ISSR数据进行组合的主成分分析（PCA）分析在散点图中将所检查的物种分为四类。PCA散点图中物种/种群的区分与其在聚类树中的分离是一致的。这四个组是1.麦加链霉菌，凝结链球菌和schimperianum。2. glabratum和S. incanum。3. S. dulcamara，S。sisymbriifolium和S. torvum，4 . S. nigrum，S。villosum和S. villosum ssp。puniceum。