Objective: This study was designed to investigate the acute effects of non-nicotinized e-cigarette (e-cig) aerosol inhalation in nonsmokers both in terms of blood-based markers of inflammation and oxidative stress and evaluate their association with hemodynamic-metabolic MRI parameters quantifying peripheral vascular reactivity, cerebrovascular reactivity and aortic stiffness. Approach and Results: Thirty-one healthy nonsmokers were subjected to two blood draws and two identical MRI protocols, each one before and after a standardized e-cig vaping session. After vaping, the serum levels of C-reactive protein (CRP), soluble intercellular adhesion molecule (sICAM-1), the danger signal machinery high mobility group box-1 (HMGB1) and its downstream effector, the NLRP3 inflammasome (as monitored by its adaptor protein ASC), increased significantly relative to the respective baseline (pre-vaping) values. Moreover, nitric oxide metabolites (NOx) and reactive oxygen species (ROS) production decreased and increased, respectively. These observations were paralleled by impaired peripheral vascular reactivity (with reduced flow mediated dilation and attenuated hyperemic response after a cuff-occlusion test), and metabolic alterations expressed by decreased venous oxygen saturation, post-vaping. Conclusion: The current results suggest propagation of inflammation signaling via activation of the danger signaling axis (HMGB1-NLRP3). The findings indicate that a single episode of vaping has adverse impacts on vascular inflammation and function.

中文翻译：

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急性电子烟吸入影响血管健康：一项针对未吸烟受试者的研究

目的：本研究旨在调查非烟碱化电子烟 (e-cig) 气溶胶吸入对非吸烟者的急性影响，包括炎症和氧化应激的血液标志物，并评估它们与血液动力学-代谢 MRI 参数的关系量化外周血管反应性、脑血管反应性和主动脉僵硬度。方法和结果：对 31 名健康的不吸烟者进行了两次抽血和两次相同的 MRI 协议，每一次都是在标准化的电子烟吸食前后进行的。抽电子烟后，血清 C 反应蛋白 (CRP)、可溶性细胞间粘附分子 (sICAM-1)、危险信号机制高迁移率基团盒 1 (HMGB1) 及其下游效应子 NLRP3 炎症小体的水平（由它的衔接蛋白ASC），相对于各自的基线（吸电子烟前）值显着增加。此外，一氧化氮代谢物 (NOx) 和活性氧 (ROS) 的产生分别减少和增加。这些观察结果与外周血管反应性受损（血流介导的扩张减少和袖带闭塞试验后充血反应减弱）以及静脉血氧饱和度降低所表达的代谢改变，电子烟后平行。结论：目前的结果表明通过激活危险信号轴 (HMGB1-NLRP3) 传播炎症信号。研究结果表明，单次吸电子烟会对血管炎症和功能产生不利影响。一氧化氮代谢物 (NOx) 和活性氧 (ROS) 的产生分别减少和增加。这些观察结果与外周血管反应性受损（血流介导的扩张减少和袖带闭塞试验后充血反应减弱）以及静脉血氧饱和度降低所表达的代谢改变，电子烟后平行。结论：目前的结果表明通过激活危险信号轴 (HMGB1-NLRP3) 传播炎症信号。研究结果表明，单次吸电子烟会对血管炎症和功能产生不利影响。一氧化氮代谢物 (NOx) 和活性氧 (ROS) 的产生分别减少和增加。这些观察结果与外周血管反应性受损（血流介导的扩张减少和袖带闭塞试验后充血反应减弱）以及静脉血氧饱和度降低所表达的代谢改变，电子烟后平行。结论：目前的结果表明通过激活危险信号轴 (HMGB1-NLRP3) 传播炎症信号。研究结果表明，单次吸电子烟会对血管炎症和功能产生不利影响。和代谢改变，表现为吸电子烟后静脉血氧饱和度降低。结论：目前的结果表明通过激活危险信号轴 (HMGB1-NLRP3) 传播炎症信号。研究结果表明，单次吸电子烟会对血管炎症和功能产生不利影响。和代谢改变，表现为吸电子烟后静脉血氧饱和度降低。结论：目前的结果表明通过激活危险信号轴 (HMGB1-NLRP3) 传播炎症信号。研究结果表明，单次吸电子烟会对血管炎症和功能产生不利影响。